Activation of the Akt/GSK3β signaling pathway mediates survival of vulnerable hippocampal neurons after transient global cerebral ischemia in rats

Hidenori Endo, Chikako Nito, Hiroshi Kamada, Tatsuro Nishi, Pak H. Chan

Research output: Contribution to journalArticlepeer-review

146 Citations (Scopus)

Abstract

Recent studies have revealed that the phosphatidylinositol 3-kinase (PI3-K) pathway is involved in apoptotic cell death after experimental cerebral ischemia. The serine-threonine kinase, Akt, functions in the PI3-K pathway and prevents apoptosis by phosphorylation at Ser473 after a variety of cell death stimuli. After phosphorylation, activated Akt inactivates other apoptogenic factors, including glycogen synthase kinase-3β (GSK3β), thereby inhibiting cell death. However, the role of Akt/GSK3β signaling in the delayed death of hippocampal neurons in the CA1 subregion after transient global cerebral ischemia (tGCI) has not been clarified. Transient global cerebral ischemia for 5 mins was induced by bilateral common carotid artery occlusion combined with hypotension. Western blot analysis showed a significant increase in phospho-Akt (Ser473) and phospho-GSK3β (Ser9) in the hippocampal CA1 subregion after tGCI. Immunohistochemistry showed that expression of phospho-Akt (Ser473) and phospho-GSK3β (Ser9) was markedly increased in the vulnerable CA1 subregion, but not in the ischemic-tolerant CA3 subregion. Double staining with phospho-GSK3β (Ser9) and terminal deoxynucleotidyl transferase-mediated uridine 5′-triphosphate-biotin nick end labeling showed different cellular distributions in the CA1 subregion 3 days after tGCI. Phosphorylation of Akt and GSK3β was prevented by LY294002, a PI3-K inhibitor, which facilitated subsequent DNA fragmentation 3 days after tGCI. Moreover, transgenic rats that overexpress copper/zinc-superoxide dismutase, which is known to be neuroprotective against delayed hippocampal CA1 injury after tGCI, had enhanced and persistent phosphorylation of both Akt and GSK3β after tGCI. These findings suggest that activation of the Akt/GSK3β signaling pathway may mediate survival of vulnerable hippocampal CA1 neurons after tGCI.

Original languageEnglish
Pages (from-to)1479-1489
Number of pages11
JournalJournal of Cerebral Blood Flow and Metabolism
Volume26
Issue number12
DOIs
Publication statusPublished - 2006 Dec 8

Keywords

  • Akt
  • Apoptosis
  • GSK3b
  • PI3-K pathway
  • Superoxide dismutase
  • Transient global cerebral ischemia

ASJC Scopus subject areas

  • Neurology
  • Clinical Neurology
  • Cardiology and Cardiovascular Medicine

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