Activation of phospholipase a from pseudomonas aeruginosa by ammonium sulfate and heat treatment

Tomonobu Kusano; Kazuo Izaki; Hajime Takahashi

doi:10.1080/00021369.1980.10864210

Activation of phospholipase a from pseudomonas aeruginosa by ammonium sulfate and heat treatment

Tomonobu Kusano, Kazuo Izaki, Hajime Takahashi

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

Phospholipase A activity of Pseudomonas aeruginosa was found only in the membrane fraction under normal conditions, but a part of that activity was converted into soluble form after polymyxin B treatment. This soluble phospholipase A was further activated by addition of 80% saturated (NH₄)₂SO₄or incubation at 70 ± 5°C for 5 minutes. The phenomenon was confirmed in terms of kinetic parameters. Phospholipase A activity in the sonicates of P aeruginosa was also markedly enhanced by (NH₄)₂SO₂at 80% saturated concentration. The mechanism of the activation is discussed.

Original language	English
Pages (from-to)	1779-1785
Number of pages	7
Journal	Agricultural and Biological Chemistry
Volume	44
Issue number	8
DOIs	https://doi.org/10.1080/00021369.1980.10864210
Publication status	Published - 1980 Aug

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)
Agricultural and Biological Sciences(all)

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10.1080/00021369.1980.10864210

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title = "Activation of phospholipase a from pseudomonas aeruginosa by ammonium sulfate and heat treatment",

abstract = "Phospholipase A activity of Pseudomonas aeruginosa was found only in the membrane fraction under normal conditions, but a part of that activity was converted into soluble form after polymyxin B treatment. This soluble phospholipase A was further activated by addition of 80% saturated (NH4)2SO4or incubation at 70 ± 5°C for 5 minutes. The phenomenon was confirmed in terms of kinetic parameters. Phospholipase A activity in the sonicates of P aeruginosa was also markedly enhanced by (NH4)2SO2at 80% saturated concentration. The mechanism of the activation is discussed.",

author = "Tomonobu Kusano and Kazuo Izaki and Hajime Takahashi",

note = "Funding Information: Acknowledgments. The authors wish to thank Miss Aiko Sato for her operation of the liquid scintillation spectrometer. This investigation was supported in part by a Grant-inAid for Scientific Research from the Ministry of Education, Science and Culture (to H. T. 443026).",

year = "1980",

month = aug,

doi = "10.1080/00021369.1980.10864210",

language = "English",

volume = "44",

pages = "1779--1785",

journal = "Bioscience, Biotechnology and Biochemistry",

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publisher = "Japan Society for Bioscience Biotechnology and Agrochemistry",

number = "8",

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T1 - Activation of phospholipase a from pseudomonas aeruginosa by ammonium sulfate and heat treatment

AU - Kusano, Tomonobu

AU - Izaki, Kazuo

AU - Takahashi, Hajime

N1 - Funding Information: Acknowledgments. The authors wish to thank Miss Aiko Sato for her operation of the liquid scintillation spectrometer. This investigation was supported in part by a Grant-inAid for Scientific Research from the Ministry of Education, Science and Culture (to H. T. 443026).

PY - 1980/8

Y1 - 1980/8

N2 - Phospholipase A activity of Pseudomonas aeruginosa was found only in the membrane fraction under normal conditions, but a part of that activity was converted into soluble form after polymyxin B treatment. This soluble phospholipase A was further activated by addition of 80% saturated (NH4)2SO4or incubation at 70 ± 5°C for 5 minutes. The phenomenon was confirmed in terms of kinetic parameters. Phospholipase A activity in the sonicates of P aeruginosa was also markedly enhanced by (NH4)2SO2at 80% saturated concentration. The mechanism of the activation is discussed.

AB - Phospholipase A activity of Pseudomonas aeruginosa was found only in the membrane fraction under normal conditions, but a part of that activity was converted into soluble form after polymyxin B treatment. This soluble phospholipase A was further activated by addition of 80% saturated (NH4)2SO4or incubation at 70 ± 5°C for 5 minutes. The phenomenon was confirmed in terms of kinetic parameters. Phospholipase A activity in the sonicates of P aeruginosa was also markedly enhanced by (NH4)2SO2at 80% saturated concentration. The mechanism of the activation is discussed.

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Activation of phospholipase a from pseudomonas aeruginosa by ammonium sulfate and heat treatment

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