Activation of phospholipase a from pseudomonas aeruginosa by ammonium sulfate and heat treatment

Tomonobu Kusano; Kazuo Izaki; Hajime Takahashi

doi:10.1080/00021369.1980.10864210

Activation of phospholipase a from pseudomonas aeruginosa by ammonium sulfate and heat treatment

Tomonobu Kusano, Kazuo Izaki, Hajime Takahashi

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

Phospholipase A activity of Pseudomonas aeruginosa was found only in the membrane fraction under normal conditions, but a part of that activity was converted into soluble form after polymyxin B treatment. This soluble phospholipase A was further activated by addition of 80% saturated (NH₄)₂SO₄or incubation at 70 ± 5°C for 5 minutes. The phenomenon was confirmed in terms of kinetic parameters. Phospholipase A activity in the sonicates of P aeruginosa was also markedly enhanced by (NH₄)₂SO₂at 80% saturated concentration. The mechanism of the activation is discussed.

Original language	English
Pages (from-to)	1779-1785
Number of pages	7
Journal	Agricultural and Biological Chemistry
Volume	44
Issue number	8
DOIs	https://doi.org/10.1080/00021369.1980.10864210
Publication status	Published - 1980 Aug

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)
Agricultural and Biological Sciences(all)

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abstract = "Phospholipase A activity of Pseudomonas aeruginosa was found only in the membrane fraction under normal conditions, but a part of that activity was converted into soluble form after polymyxin B treatment. This soluble phospholipase A was further activated by addition of 80% saturated (NH4)2SO4or incubation at 70 ± 5°C for 5 minutes. The phenomenon was confirmed in terms of kinetic parameters. Phospholipase A activity in the sonicates of P aeruginosa was also markedly enhanced by (NH4)2SO2at 80% saturated concentration. The mechanism of the activation is discussed.",

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AU - Kusano, Tomonobu

AU - Izaki, Kazuo

AU - Takahashi, Hajime

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N2 - Phospholipase A activity of Pseudomonas aeruginosa was found only in the membrane fraction under normal conditions, but a part of that activity was converted into soluble form after polymyxin B treatment. This soluble phospholipase A was further activated by addition of 80% saturated (NH4)2SO4or incubation at 70 ± 5°C for 5 minutes. The phenomenon was confirmed in terms of kinetic parameters. Phospholipase A activity in the sonicates of P aeruginosa was also markedly enhanced by (NH4)2SO2at 80% saturated concentration. The mechanism of the activation is discussed.

AB - Phospholipase A activity of Pseudomonas aeruginosa was found only in the membrane fraction under normal conditions, but a part of that activity was converted into soluble form after polymyxin B treatment. This soluble phospholipase A was further activated by addition of 80% saturated (NH4)2SO4or incubation at 70 ± 5°C for 5 minutes. The phenomenon was confirmed in terms of kinetic parameters. Phospholipase A activity in the sonicates of P aeruginosa was also markedly enhanced by (NH4)2SO2at 80% saturated concentration. The mechanism of the activation is discussed.

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