TY - JOUR
T1 - Activation of phospholipase a from pseudomonas aeruginosa by ammonium sulfate and heat treatment
AU - Kusano, Tomonobu
AU - Izaki, Kazuo
AU - Takahashi, Hajime
N1 - Funding Information:
Acknowledgments. The authors wish to thank Miss Aiko Sato for her operation of the liquid scintillation spectrometer. This investigation was supported in part by a Grant-inAid for Scientific Research from the Ministry of Education, Science and Culture (to H. T. 443026).
PY - 1980/8
Y1 - 1980/8
N2 - Phospholipase A activity of Pseudomonas aeruginosa was found only in the membrane fraction under normal conditions, but a part of that activity was converted into soluble form after polymyxin B treatment. This soluble phospholipase A was further activated by addition of 80% saturated (NH4)2SO4or incubation at 70 ± 5°C for 5 minutes. The phenomenon was confirmed in terms of kinetic parameters. Phospholipase A activity in the sonicates of P aeruginosa was also markedly enhanced by (NH4)2SO2at 80% saturated concentration. The mechanism of the activation is discussed.
AB - Phospholipase A activity of Pseudomonas aeruginosa was found only in the membrane fraction under normal conditions, but a part of that activity was converted into soluble form after polymyxin B treatment. This soluble phospholipase A was further activated by addition of 80% saturated (NH4)2SO4or incubation at 70 ± 5°C for 5 minutes. The phenomenon was confirmed in terms of kinetic parameters. Phospholipase A activity in the sonicates of P aeruginosa was also markedly enhanced by (NH4)2SO2at 80% saturated concentration. The mechanism of the activation is discussed.
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U2 - 10.1080/00021369.1980.10864210
DO - 10.1080/00021369.1980.10864210
M3 - Article
AN - SCOPUS:84954890094
VL - 44
SP - 1779
EP - 1785
JO - Bioscience, Biotechnology and Biochemistry
JF - Bioscience, Biotechnology and Biochemistry
SN - 0916-8451
IS - 8
ER -