Activation of phospholipase a from pseudomonas aeruginosa by ammonium sulfate and heat treatment

Tomonobu Kusano, Kazuo Izaki, Hajime Takahashi

    Research output: Contribution to journalArticlepeer-review

    3 Citations (Scopus)

    Abstract

    Phospholipase A activity of Pseudomonas aeruginosa was found only in the membrane fraction under normal conditions, but a part of that activity was converted into soluble form after polymyxin B treatment. This soluble phospholipase A was further activated by addition of 80% saturated (NH4)2SO4or incubation at 70 ± 5°C for 5 minutes. The phenomenon was confirmed in terms of kinetic parameters. Phospholipase A activity in the sonicates of P aeruginosa was also markedly enhanced by (NH4)2SO2at 80% saturated concentration. The mechanism of the activation is discussed.

    Original languageEnglish
    Pages (from-to)1779-1785
    Number of pages7
    JournalAgricultural and Biological Chemistry
    Volume44
    Issue number8
    DOIs
    Publication statusPublished - 1980 Aug

    ASJC Scopus subject areas

    • Biochemistry, Genetics and Molecular Biology(all)
    • Agricultural and Biological Sciences(all)

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