Activation of myeloid dendritic cells by deoxynucleic acids from Cordyceps sinensis via a Toll-like receptor 9-dependent pathway

Gang Xiao, Akiko Miyazato, Yuzuru Abe, Tiantuo Zhang, Kiwamu Nakamura, Ken Inden, Misuzu Tanaka, Daiki Tanno, Tomomitsu Miyasaka, Keiko Ishii, Kiyoshi Takeda, Shizuo Akira, Shinobu Saijo, Yoichiro Iwakura, Yoshiyuki Adachi, Naohito Ohno, Natsuo Yamamoto, Hiroyuki Kunishima, Yoichi Hirakata, Mitsuo KakuKazuyoshi Kawakami

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)

Abstract

The mechanism by which host cells recognize Cordyceps sinensis, a Chinese herbal medicine that is known to exhibit immunomodulating activity, remains poorly understood. In this study, we investigated whether the DNA of this fungus could activate mouse bone marrow-derived dendritic cells (BM-DCs). Upon stimulation with C. sinensis DNA, BM-DCs released IL-12p40 and TNF-α and expressed CD40. Cytokine production and CD40 expression were attenuated by chloroquin and bafilomycin A. Activation of BM-DCs by C. sinensis DNA was almost completely abrogated in TLR9KO mice. According to a luciferase reporter assay, C. sinensis DNA activated NF-κB in HEK293T cells transfected with the TLR9 gene. Finally, a confocal microscopic analysis showed that C. sinensis DNA was co-localized with CpG-ODN and partly with TLR9 and LAMP-1, a late endosomal marker, in BM-DCs. Our results demonstrated that C. sinensis DNA caused activation of BM-DCs in a TLR9-dependent manner.

Original languageEnglish
Pages (from-to)241-250
Number of pages10
JournalCellular Immunology
Volume263
Issue number2
DOIs
Publication statusPublished - 2010

Keywords

  • CD40
  • Cordyceps sinensis
  • Cytokines
  • DNA
  • Dendritic cells
  • TLR9

ASJC Scopus subject areas

  • Immunology

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