Activation of EGFR, AKT and ERK1/2 by exposure to tritiated water in human tumor cells

Tsutomu Shimura, Yoshikazu Kuwahara, Manabu Fukumoto, Toshiyuki Umata

Research output: Contribution to journalArticlepeer-review

Abstract

Tritium is used as fuel for nuclear fusion reactions and has cancer risk for workers in nuclear fusion plant by its exposure during routine operation or accidents. Tritium exists as tritiated water (HTO) which is produced by an isotope exchange reaction from HT in normal circumstance. In this study we analyzed cellular response against HTO exposure in a human liver cancer cell line HepG2 and a cervical cancer cell line HeLa. These cells were immersed in RPMI medium containing HTO at the dose rate of 0.5 Gy/hrfor 20 hrs (10 Gy). We determined whether the DNA-PK/AKT pathway is activated by chronic HTO exposure or not. As well as X-rays, exposure of HepG2 and HeLa cells to HTO activated AKT, which was shown by phosphorylated-AKT at Serine473. The activation of DNA-PK was also observed in these cells by X-ray irradiation but not by exposure to HTO. Instead of DNA-PK, epidermal growth factor receptor and ERKl/2 were activated following HTO exposure. These results suggested that certain cellular molecules are response to HTO exposure. Our study may provide molecular markers to estimate the biological effects in response to tritium exposure in human cells.

Original languageEnglish
Pages (from-to)1190-1192
Number of pages3
JournalFusion Science and Technology
Volume60
Issue number3
DOIs
Publication statusPublished - 2011 Oct

ASJC Scopus subject areas

  • Civil and Structural Engineering
  • Nuclear and High Energy Physics
  • Nuclear Energy and Engineering
  • Materials Science(all)
  • Mechanical Engineering

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