Activation of Ca2+/calmodulin-dependent protein kinase II in cerebellar granule cells by N-methyl-d-aspartate receptor activation

Koji Fukunaga, Thomas R. Soderling

Research output: Contribution to journalArticlepeer-review

36 Citations (Scopus)

Abstract

Cultured cerebellar granule cells were studied to determine if the excitatory neurotransmitter glutamate acting through the N-methyl-d-aspartate (NMDA) receptor could stimulate autophosphorylation of Ca2+/calmodulin-dependent protein kinase II (CaM-kinase II) to generate its Ca2+-independent form. Glutamate did elevate Ca2+-independent CaM-kinase II through autophosphorylation when granule cells were incubated in Mg2+-free buffer, and this response was potentiated by 1 μM glycine. Extracellular Ca2+ was required, and specific antagonists of the NMDA receptor blocked the response. These results support the hypothesis that postsynaptic Ca2+ influx through the NMDA receptor-gated ion channel, as occurs during induction of long-term potentiation, may convert CaM-kinase II to a constitutively active, Ca2+-independent form.

Original languageEnglish
Pages (from-to)133-138
Number of pages6
JournalMolecular and Cellular Neuroscience
Volume1
Issue number2
DOIs
Publication statusPublished - 1990 Oct
Externally publishedYes

ASJC Scopus subject areas

  • Molecular Biology
  • Cellular and Molecular Neuroscience
  • Cell Biology

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