TY - JOUR
T1 - Activation of Ca2+/calmodulin-dependent protein kinase II by stimulation with bradykinin in neuroblastoma x glioma hybrid NG108-15 cells
AU - Yamakawa, Takashi
AU - Fukunaga, Kohji
AU - Higashida, Haruhiro
AU - Miyamoto, Eishichi
N1 - Funding Information:
Acknowledgements. This work was supported in part by a Grant-in-Aid for Scientific Research from the Ministry of Education, Science and Culture of Japan.
PY - 1992/12/4
Y1 - 1992/12/4
N2 - To elucidate the mechanisms of the initracellular signal transduction elicited with bradykinin in NG108-15 neuroblastoma x glioma hybrid cells, we examined the activation of Ca2+/calmodulin-dependent protein kinase II (CaM kinase II) by bradykinin stimulation. When the extract of NG108-15 cells was immunoprecipitated with the affinity-purified antibody to brain CaM kinase II, a 50-kDa protein in the immunoprecipitate mainly became autophosphorylated in a Ca2+/calmodulin-dependent manner. The result suggest that the 50-kDa protein is the subunit of CaM kinasee II in NG108-15 cells.The Ca2+/calmodulin-independent activity (autonomous activity) of the enzyme increased twice within 10 s by stimulation with 1 μM bradykinin in the cells. The increase in the autonomous activity of the enzyme had two phases: the transient early-peak phase and the long late-plateau phase. The former was abolished by the pretreatment of the cells with 10 mM caffeine or 20 μM BAPTA-AM, and the latter was abolished by the removal of the extracellular Ca2+ with 1 mM EGTA or by the pretreatment with 1 μM nifedipine. Stimulation of 32P-labeled NG108-15 cells with 1 μM bradykinin increased the autophosphorylation of CaM kinase II and this increase was abolished by pretreatment with caffeine or BAPTA-AM. Theseesults suggest that CaM kinase II is activated via the inositol phospholipid signaling pathway induced with bradykinin in NG108-15 cells.
AB - To elucidate the mechanisms of the initracellular signal transduction elicited with bradykinin in NG108-15 neuroblastoma x glioma hybrid cells, we examined the activation of Ca2+/calmodulin-dependent protein kinase II (CaM kinase II) by bradykinin stimulation. When the extract of NG108-15 cells was immunoprecipitated with the affinity-purified antibody to brain CaM kinase II, a 50-kDa protein in the immunoprecipitate mainly became autophosphorylated in a Ca2+/calmodulin-dependent manner. The result suggest that the 50-kDa protein is the subunit of CaM kinasee II in NG108-15 cells.The Ca2+/calmodulin-independent activity (autonomous activity) of the enzyme increased twice within 10 s by stimulation with 1 μM bradykinin in the cells. The increase in the autonomous activity of the enzyme had two phases: the transient early-peak phase and the long late-plateau phase. The former was abolished by the pretreatment of the cells with 10 mM caffeine or 20 μM BAPTA-AM, and the latter was abolished by the removal of the extracellular Ca2+ with 1 mM EGTA or by the pretreatment with 1 μM nifedipine. Stimulation of 32P-labeled NG108-15 cells with 1 μM bradykinin increased the autophosphorylation of CaM kinase II and this increase was abolished by pretreatment with caffeine or BAPTA-AM. Theseesults suggest that CaM kinase II is activated via the inositol phospholipid signaling pathway induced with bradykinin in NG108-15 cells.
KW - Bradykinin
KW - Ca/calmodulin-dependent protein kinase II
KW - Inositol phospholipid metabolism
KW - Intracellular Ca
KW - NG108-15 neuroblastoma x glioma hybrid cell
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U2 - 10.1016/0006-8993(92)91477-V
DO - 10.1016/0006-8993(92)91477-V
M3 - Article
C2 - 1335347
AN - SCOPUS:0026615761
VL - 597
SP - 220
EP - 226
JO - Molecular Brain Research
JF - Molecular Brain Research
SN - 0006-8993
IS - 2
ER -