Activation of c-Jun amino-terminal kinase is required for retinoic acid-induced neural differentiation of P19 embryonal carcinoma cells

Hong Wang, Shoko Ikeda, Shin ichiro Kanno, Li Ming Guang, Motoko Ohnishi, Masato Sasaki, Takayasu Kobayashi, Shinri Tamura

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

P19 embryonal carcinoma cells are known to differentiate into neurons and glia when treated with relatively high concentrations ( > 100 nM) of retinoic acid (RA). Concomitant with this RA-induced neural differentiation, we observed an activation of the c-Jun amino-terminal kinase (JNK). JNK was required for the RA-induced neural differentiation, because dominant-negative JNK blocked the differentiation. Studies using protein phosphatase inhibitors and protein kinase inhibitors suggested that both okadaic acid-sensitive protein phosphatase(s) and protein kinase C participate in the RA-induced activation of JNK.

Original languageEnglish
Pages (from-to)91-96
Number of pages6
JournalFEBS Letters
Volume503
Issue number1
DOIs
Publication statusPublished - 2001 Aug 10

Keywords

  • C-Jun amino-terminal kinase
  • Neural differentiation
  • Okadaic acid-sensitive protein phosphatase
  • P19 embryonal carcinoma cell
  • Protein kinase C
  • Retinoic acid

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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