Activation of adenosine receptor on gingival fibroblasts

T. Hashikawa, M. Takedachi, M. Terakura, S. Yamada, L. F. Thompson, Y. Shimabukuro, S. Murakami

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)


CD73 (ecto-5′-nucleotidase) on human gingival fibroblasts plays a role in the regulation of intracellular cAMP levels through the generation of adenosine, which subsequently activates adenosine receptors. In this study, we examined the involvement of ecto-adenosine deaminase, which can be anchored to CD26 on human gingival fibroblasts, in metabolizing adenosine generated by CD73, and thus attenuating adenosine receptor activation. Ecto-adenosine deaminase expression on fibroblasts could be increased by pre-treatment with a lysate of Jurkat cells, a cell line rich in cytoplasmic adenosine deaminase. Interestingly, the cAMP response to adenosine generated from 5′-AMP via CD73 and the ability of 5′-AMP to induce hyaluronan synthase 1 mRNA were significantly decreased by the pre-treatment of fibroblasts with Jurkat cell lysate. This inhibitory effect was reversed by the specific adenosine deaminase inhibitor. These results suggest that ecto-adenosine deaminase metabolizes CD73-generated adenosine and regulates adenosine receptor activation.

Original languageEnglish
Pages (from-to)739-744
Number of pages6
JournalJournal of dental research
Issue number8
Publication statusPublished - 2006 Aug
Externally publishedYes


  • Adenosine receptor
  • CD73
  • Ecto-adenosine deaminase

ASJC Scopus subject areas

  • Dentistry(all)


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