Action of aspirin on whole blood-aggregation evaluated by the screen filtration pressure method

Arata Tabuchi, Ryoji Taniguchi, Kanako Takahashi, Hirokazu Kondo, Mitsunori Kawato, Takeshi Morimoto, Takeshi Kimura, Toru Kita, Hisanori Horiuchi

Research output: Contribution to journalArticlepeer-review

15 Citations (Scopus)

Abstract

Background: There are few monitoring systems widely used in clinical practice for evaluating the effectiveness of aspirin therapy, so in the present study aspirin's antiplatelet effects we investigated with a whole blood aggregometer using a screen filtration pressure (SFP) method. Methods and Results: Thirty-five healthy male volunteers took 100 mg/day aspirin for 14 days. Whole-blood aggregation was analyzed at baseline and on days 7 and 14, using collagen and adenosine diphosphate as the stimuli, and compared with the platelet-rich plasma (PRP) aggregation measured by optical aggregometer. The platelet-aggregation threshold index (PATI) for both methods, which was defined as the putative agonist-concentration giving half-maximal aggregation, and the PRP-maximal aggregation rate were analyzed. The maximal aggregation rate induced by 1.6 mg/L collagen decreased from 85.5% (80.8-92.8) [median (interquartile range)] at baseline to 51.5% (39-63.8) on day 14 (p<0.0001). The PRP-PATI and whole-blood PATI for collagen increased from 0.32 (0.28-0.70) to 1.82 mg/L (1.25-2.89) (p<0.0001) and from 0.28 (0.22-0.3) to 1.06 mg/L (1.01-1.29) (p<0.0001) respectively. Conclusions: The whole-blood PATI and PRP-PATI for collagen, as well as the maximal PRP aggregation rate, clearly distinguish platelet aggregability before and after aspirin intake. However, whole-blood analysis by the SFP-method is easier to perform, and is a promising method of monitoring aspirin's effects.

Original languageEnglish
Pages (from-to)420-426
Number of pages7
JournalCirculation Journal
Volume72
Issue number3
DOIs
Publication statusPublished - 2008

Keywords

  • Aggregation
  • Aspirin
  • Platelet-aggregation threshold index (PATI)
  • Screen filtration pressure method
  • Whole blood-aggregation

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Fingerprint Dive into the research topics of 'Action of aspirin on whole blood-aggregation evaluated by the screen filtration pressure method'. Together they form a unique fingerprint.

Cite this