Actin-related protein Arp4 functions in kinetochore assembly

Hideaki Ogiwara, Ayako Ui, Satoshi Kawashima, Kazuto Kugou, Fumitoshi Onoda, Hitoshi Iwahashi, Masahiko Harata, Kunihiro Ohta, Takemi Enomoto, Masayuki Seki

Research output: Contribution to journalArticlepeer-review

24 Citations (Scopus)

Abstract

The actin-related proteins (Arps) comprise a conserved protein family. Arp4p is found in large multisubunits of the INO80 and SWR1 chromatin remodeling complexes and in the NuA4 histone acetyltransferase complex. Here we show that arp4 (arp4S23A/D159A) temperature-sensitive cells are defective in G2/M phase function. arp4 mutants are sensitive to the microtubule depolymerizing agent benomyl and arrest at G2/M phase at restrictive temperature. Arp4p is associated with centromeric and telomeric regions throughout cell cycle. Ino80p, Esa1p and Swr1p, components of the INO80, NuA4 and SWR1 complexes, respectively, also associate with centromeres. The association of many kinetochore components including Cse4p, a component of the centromere nucleosome, Mtw1p and Ctf3p is partially impaired in arp4 cells, suggesting that the G2/M arrest of arp4 mutant cells is due to a defect in formation of the chromosomal segregation apparatus.

Original languageEnglish
Pages (from-to)3109-3117
Number of pages9
JournalNucleic acids research
Volume35
Issue number9
DOIs
Publication statusPublished - 2007 May

ASJC Scopus subject areas

  • Genetics

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