Actin polymerization machinery in live cells as revealed by single-molecule imaging

Takahiro Tsuji, Naoki Watanabe

    Research output: Contribution to journalReview articlepeer-review

    Abstract

    Actin polymerization generates force to drive cell edge protrusion, and plays an important role in migration and shape change of cells. Many molecular mechanisms regulating actin dynamics have been identified, but probing their individual roles within the cells becomes increasingly difficult because of their complex interactions. Here we describe our invention of single-molecule visualization of fluorescently-labeled actin and its related molecules that has revealed dynamic nature of the actin arrays in live cells. Single-molecule observation enabled us to visualize the position and the duration of assembly of actin filaments and actin-related molecules. Moreover, this imaging method led us to discover processive actin-capping motion of a formin-related protein, mDial. Our approach of single-molecule imaging will be instrumental to quantitative analysis of cell signaling.

    Original languageEnglish
    Pages (from-to)235-241
    Number of pages7
    JournalNippon rinsho. Japanese journal of clinical medicine
    Volume65
    Issue number2
    Publication statusPublished - 2007 Feb

    ASJC Scopus subject areas

    • Medicine(all)

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