Actin migration driven by directional assembly and disassembly of membrane-anchored actin filaments

Hiroko Katsuno, Michinori Toriyama, Yoichiroh Hosokawa, Kensaku Mizuno, Kazushi Ikeda, Yuichi Sakumura, Naoyuki Inagaki

    Research output: Contribution to journalArticlepeer-review

    46 Citations (Scopus)

    Abstract

    Actin and actin-associated proteins migrate within various cell types. To uncover the mechanism of their migration, we analyzed actin waves, which translocate actin and actin-associated proteins along neuronal axons toward the growth cones. We found that arrays of actin filaments constituting waves undergo directional assembly and disassembly, with their polymerizing ends oriented toward the axonal tip, and that the lateral side of the filaments is mechanically anchored to the adhesive substrate. A combination of live-cell imaging, molecular manipulation, force measurement, and mathematical modeling revealed that wave migration is driven by directional assembly and disassembly of actin filaments and their anchorage to the substrate. Actinassociated proteins co-migrate with actin filaments by interacting with them. Furthermore, blocking this migration, by creating an adhesion-free gap along the axon, disrupts axonal protrusion. Our findings identify a molecular mechanism that translocates actin and associated proteins toward the cell's leading edge, thereby promoting directional cell motility.

    Original languageEnglish
    Pages (from-to)648-660
    Number of pages13
    JournalCell Reports
    Volume12
    Issue number4
    DOIs
    Publication statusPublished - 2015

    ASJC Scopus subject areas

    • Biochemistry, Genetics and Molecular Biology(all)

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