TY - JOUR
T1 - Actin gene-targeted RT-PCR could be a useful method for evaluating in vitro fungicidal activity against dermatophytes
AU - Nimura, K.
AU - Niwano, Yoshi
AU - Ishiduka, S.
AU - Kato, M.
PY - 2003
Y1 - 2003
N2 - This study examined the effects of the antifungal agents amorolfine hydrochloride (AMF) and bifonazole (BFZ) on actin mRNA expression determined by reverse transcription-polymerase chain reaction (RT-PCR) amplification, and the morphology of Trichophyton mentagrophytes. In AMF-treated cultures, the hyphal cells of T. mentagrophytes exhibited concentration- and/or time-dependent progressively degenerative morphological damage. Those cultures exhibiting severe and necrotic morphological changes (such as crushed, bent and flattened hyphal cells) did not express actin mRNA. In BFZ-treated cultures, similar morphological changes were seen in the hyphae of T. mentagrophytes, but these changes were milder than observed in the AMF-treated samples, and actin gene fragments were amplified in all of these samples. These results indicate that actin gene-targeted RT-PCR could be a useful tool for testing the susceptibility of dermatophytes to antifungal agents in vitro.
AB - This study examined the effects of the antifungal agents amorolfine hydrochloride (AMF) and bifonazole (BFZ) on actin mRNA expression determined by reverse transcription-polymerase chain reaction (RT-PCR) amplification, and the morphology of Trichophyton mentagrophytes. In AMF-treated cultures, the hyphal cells of T. mentagrophytes exhibited concentration- and/or time-dependent progressively degenerative morphological damage. Those cultures exhibiting severe and necrotic morphological changes (such as crushed, bent and flattened hyphal cells) did not express actin mRNA. In BFZ-treated cultures, similar morphological changes were seen in the hyphae of T. mentagrophytes, but these changes were milder than observed in the AMF-treated samples, and actin gene fragments were amplified in all of these samples. These results indicate that actin gene-targeted RT-PCR could be a useful tool for testing the susceptibility of dermatophytes to antifungal agents in vitro.
KW - Actin RT-PCR
KW - Amorolfine hydrochloride
KW - Bifonazole
KW - Dermatophyte
KW - Scanning electron microscopy
UR - http://www.scopus.com/inward/record.url?scp=0142149238&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0142149238&partnerID=8YFLogxK
U2 - 10.1177/147323000303100508
DO - 10.1177/147323000303100508
M3 - Article
C2 - 14587308
AN - SCOPUS:0142149238
VL - 31
SP - 407
EP - 412
JO - Journal of International Medical Research
JF - Journal of International Medical Research
SN - 0300-0605
IS - 5
ER -