Actin gene-targeted RT-PCR could be a useful method for evaluating in vitro fungicidal activity against dermatophytes

K. Nimura, Yoshi Niwano, S. Ishiduka, M. Kato

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

This study examined the effects of the antifungal agents amorolfine hydrochloride (AMF) and bifonazole (BFZ) on actin mRNA expression determined by reverse transcription-polymerase chain reaction (RT-PCR) amplification, and the morphology of Trichophyton mentagrophytes. In AMF-treated cultures, the hyphal cells of T. mentagrophytes exhibited concentration- and/or time-dependent progressively degenerative morphological damage. Those cultures exhibiting severe and necrotic morphological changes (such as crushed, bent and flattened hyphal cells) did not express actin mRNA. In BFZ-treated cultures, similar morphological changes were seen in the hyphae of T. mentagrophytes, but these changes were milder than observed in the AMF-treated samples, and actin gene fragments were amplified in all of these samples. These results indicate that actin gene-targeted RT-PCR could be a useful tool for testing the susceptibility of dermatophytes to antifungal agents in vitro.

Original languageEnglish
Pages (from-to)407-412
Number of pages6
JournalJournal of International Medical Research
Volume31
Issue number5
DOIs
Publication statusPublished - 2003

Keywords

  • Actin RT-PCR
  • Amorolfine hydrochloride
  • Bifonazole
  • Dermatophyte
  • Scanning electron microscopy

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Biochemistry, medical

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