Acrolein, an α,β-unsaturated carbonyl, inhibits both growth and PSII activity in the cyanobacterium synechocystis sp. PCC 6803

Ginga Shimakawa, Tatsuya Iwamoto, Tomohito Mabuchi, Ryota Saito, Hiroshi Yamamoto, Katsumi Amako, Toshio Sugimoto, Amane Makino, Chikahiro Miyake

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)

Abstract

In this study, we sought to determine whether and how an α,β-unsaturated carbonyl, acrolein, can inhibit the growth of the cyanobacterium Synechocystis sp. PCC6803 (S. 6803). Treatment of S. 6803 with 200μM acrolein for 3 d significantly and irreversibly inhibited its growth. To elucidate the inhibitory mechanism, we examined the effects of acrolein on photosynthesis. In contrast to dark conditions, the addition of acrolein to S. 6803 under conditions of illumination lowered the CO2- dependent O2 evolution rate (photosynthetic activity). Furthermore, treatment with acrolein lowered the activity reducing dimethyl benzoquinone in photosystem II (PSII). Acrolein also suppressed the reduction rate for the oxidized form of the reaction center chlorophyll of photosystem I (PSI), P700. These results indicate that acrolein inhibited PSII activity in thylakoid membranes. The addition of 200μM acrolein to the illuminated S. 6803 cells gradually increased the steady-state level (Fs) of Chl fluorescence and decreased the quantum yield of PSII. These results suggested that acrolein damaged the acceptor side of PSII. On the other hand, acrolein did not inhibit respiration. From the above results, we gained insight into the metabolism of acrolein and its physiological effects in S. 6803.

Original languageEnglish
Pages (from-to)1655-1660
Number of pages6
JournalBioscience, Biotechnology and Biochemistry
Volume77
Issue number8
DOIs
Publication statusPublished - 2013 Sep 16

Keywords

  • Acrolein
  • NADPH-dependent acrolein reduction (NAR)
  • Photosynthesis
  • Plant diabetes
  • Reactive carbonyls (RCs)

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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