Acquisition of growth autonomy and tumorigenicity by an interleukin 6-dependent human myeloma cell line transfected with interleukin 6 cDNA

Y. Okuno, T. Takahashi, A. Suzuki, M. Fukumoto, K. Nakamura, H. Fukui, Y. Koishihara, Y. Ohsugi, H. Imura

Research output: Contribution to journalArticlepeer-review

22 Citations (Scopus)

Abstract

In human multiple myeloma, an autocrine growth mechanism through interleukin 6 (IL-6) has been advocated. However, growth of myeloma cells in vitro is poor except for established cell lines, and IL-6 autocrine growth is quite rare in myeloma cell lines. In the present study, we devised a model of IL-6 autocrine growth in vitro by transfecting IL-6 cDNA into a human myeloma cell line that had a proliferative response to IL-6 but did not produce IL-6. After IL-6 transfection, the cells proliferated in culture media without IL-6, and their growth rate was elevated at higher cell densities. IL-6 was detected by enzyme-linked immunosorbent assay in the culture media of the transfectants. IL-6 mRNA was distinctly expressed in these cells when analyzed by Northern blotting. The growth of the transfectants was definitely inhibited by anti-IL-6 or anti-IL-6 receptor monoclonal antibodies. Furthermore, the transfectants were successfully transplanted to nude mice. These results indicate that the myeloma cells obtained growth autonomy in vitro through IL-6 and tumorigenicity in vivo, after IL-6 transfection.

Original languageEnglish
Pages (from-to)395-400
Number of pages6
JournalExperimental Hematology
Volume20
Issue number4
Publication statusPublished - 1992 Jan 1

Keywords

  • IL-6
  • autocrine growth
  • human myeloma cells
  • transfection
  • tumorigenicity

ASJC Scopus subject areas

  • Molecular Biology
  • Hematology
  • Genetics
  • Cell Biology
  • Cancer Research

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