Acorn barnacle Megabalanus rosa lectin (BRA-3): cDNA cloning, gene structure and seasonal changes of mRNA and protein levels

Takamatsu Nobuhiko, Takeda Tadashi, Kojima Miho, Heishi Masayuki, Muramoto Koji, Kamiya Hisao, Shiba Tadayoshi

Research output: Contribution to journalArticlepeer-review

18 Citations (Scopus)

Abstract

We have isolated cDNA clones coding for a lectin (BRA-3) from the acorn barnacle, Megabalanus rosa. Sequence comparison of the cDNA clones has revealed polymorphism in the BRA-3 mRNA, which results from single-nucleotide (nt) differences at three positions. All three differences are within the coding region and cause conservative amino acid (aa) changes. The BRA-3 gene is composed of four exons, and the three single-nt differences are located on different exons. In addition, the BRA-3 mRNA and BRA-3 protein levels increased during early summer in a similar fashion, indicating that BRA-3 production is regulated mainly at the level of transcription.

Original languageEnglish
Pages (from-to)251-255
Number of pages5
JournalGene
Volume128
Issue number2
DOIs
Publication statusPublished - 1993 Jun 30
Externally publishedYes

Keywords

  • Carbohydrate recognition domain
  • exon/intron structure
  • gene expression
  • marine invertebrate
  • polymerase chain reaction
  • polymorphism
  • recombinant DNA

ASJC Scopus subject areas

  • Genetics

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