TY - JOUR
T1 - Acorn barnacle Megabalanus rosa lectin (BRA-3)
T2 - cDNA cloning, gene structure and seasonal changes of mRNA and protein levels
AU - Nobuhiko, Takamatsu
AU - Tadashi, Takeda
AU - Miho, Kojima
AU - Masayuki, Heishi
AU - Koji, Muramoto
AU - Hisao, Kamiya
AU - Tadayoshi, Shiba
N1 - Funding Information:
We thank C. Segawa, R. Toyonaga, S. Tsuto, T. Uematsua nd A. Yamashitaf or excellentt echnicala ssis-tance.T his work was supportedi n part by grants-in-aid from the Ministry of Education,S ciencea nd Culture of Japan.
PY - 1993/6/30
Y1 - 1993/6/30
N2 - We have isolated cDNA clones coding for a lectin (BRA-3) from the acorn barnacle, Megabalanus rosa. Sequence comparison of the cDNA clones has revealed polymorphism in the BRA-3 mRNA, which results from single-nucleotide (nt) differences at three positions. All three differences are within the coding region and cause conservative amino acid (aa) changes. The BRA-3 gene is composed of four exons, and the three single-nt differences are located on different exons. In addition, the BRA-3 mRNA and BRA-3 protein levels increased during early summer in a similar fashion, indicating that BRA-3 production is regulated mainly at the level of transcription.
AB - We have isolated cDNA clones coding for a lectin (BRA-3) from the acorn barnacle, Megabalanus rosa. Sequence comparison of the cDNA clones has revealed polymorphism in the BRA-3 mRNA, which results from single-nucleotide (nt) differences at three positions. All three differences are within the coding region and cause conservative amino acid (aa) changes. The BRA-3 gene is composed of four exons, and the three single-nt differences are located on different exons. In addition, the BRA-3 mRNA and BRA-3 protein levels increased during early summer in a similar fashion, indicating that BRA-3 production is regulated mainly at the level of transcription.
KW - Carbohydrate recognition domain
KW - exon/intron structure
KW - gene expression
KW - marine invertebrate
KW - polymerase chain reaction
KW - polymorphism
KW - recombinant DNA
UR - http://www.scopus.com/inward/record.url?scp=0027280760&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0027280760&partnerID=8YFLogxK
U2 - 10.1016/0378-1119(93)90570-S
DO - 10.1016/0378-1119(93)90570-S
M3 - Article
C2 - 8514190
AN - SCOPUS:0027280760
VL - 128
SP - 251
EP - 255
JO - Gene
JF - Gene
SN - 0378-1119
IS - 2
ER -