Acorn barnacle Megabalanus rosa lectin (BRA-3): cDNA cloning, gene structure and seasonal changes of mRNA and protein levels

Takamatsu Nobuhiko; Takeda Tadashi; Kojima Miho; Heishi Masayuki; Muramoto Koji; Kamiya Hisao; Shiba Tadayoshi

doi:10.1016/0378-1119(93)90570-S

Acorn barnacle Megabalanus rosa lectin (BRA-3): cDNA cloning, gene structure and seasonal changes of mRNA and protein levels

Takamatsu Nobuhiko, Takeda Tadashi, Kojima Miho, Heishi Masayuki, Muramoto Koji, Kamiya Hisao, Shiba Tadayoshi

Research output: Contribution to journal › Article › peer-review

18 Citations (Scopus)

Abstract

We have isolated cDNA clones coding for a lectin (BRA-3) from the acorn barnacle, Megabalanus rosa. Sequence comparison of the cDNA clones has revealed polymorphism in the BRA-3 mRNA, which results from single-nucleotide (nt) differences at three positions. All three differences are within the coding region and cause conservative amino acid (aa) changes. The BRA-3 gene is composed of four exons, and the three single-nt differences are located on different exons. In addition, the BRA-3 mRNA and BRA-3 protein levels increased during early summer in a similar fashion, indicating that BRA-3 production is regulated mainly at the level of transcription.

Original language	English
Pages (from-to)	251-255
Number of pages	5
Journal	Gene
Volume	128
Issue number	2
DOIs	https://doi.org/10.1016/0378-1119(93)90570-S
Publication status	Published - 1993 Jun 30
Externally published	Yes

Keywords

Carbohydrate recognition domain
exon/intron structure
gene expression
marine invertebrate
polymerase chain reaction
polymorphism
recombinant DNA

ASJC Scopus subject areas

Genetics

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10.1016/0378-1119(93)90570-S

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title = "Acorn barnacle Megabalanus rosa lectin (BRA-3): cDNA cloning, gene structure and seasonal changes of mRNA and protein levels",

abstract = "We have isolated cDNA clones coding for a lectin (BRA-3) from the acorn barnacle, Megabalanus rosa. Sequence comparison of the cDNA clones has revealed polymorphism in the BRA-3 mRNA, which results from single-nucleotide (nt) differences at three positions. All three differences are within the coding region and cause conservative amino acid (aa) changes. The BRA-3 gene is composed of four exons, and the three single-nt differences are located on different exons. In addition, the BRA-3 mRNA and BRA-3 protein levels increased during early summer in a similar fashion, indicating that BRA-3 production is regulated mainly at the level of transcription.",

keywords = "Carbohydrate recognition domain, exon/intron structure, gene expression, marine invertebrate, polymerase chain reaction, polymorphism, recombinant DNA",

author = "Takamatsu Nobuhiko and Takeda Tadashi and Kojima Miho and Heishi Masayuki and Muramoto Koji and Kamiya Hisao and Shiba Tadayoshi",

note = "Funding Information: We thank C. Segawa, R. Toyonaga, S. Tsuto, T. Uematsua nd A. Yamashitaf or excellentt echnicala ssis-tance.T his work was supportedi n part by grants-in-aid from the Ministry of Education,S ciencea nd Culture of Japan.",

year = "1993",

month = jun,

day = "30",

doi = "10.1016/0378-1119(93)90570-S",

language = "English",

volume = "128",

pages = "251--255",

journal = "Gene",

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TY - JOUR

T1 - Acorn barnacle Megabalanus rosa lectin (BRA-3)

T2 - cDNA cloning, gene structure and seasonal changes of mRNA and protein levels

AU - Nobuhiko, Takamatsu

AU - Tadashi, Takeda

AU - Miho, Kojima

AU - Masayuki, Heishi

AU - Koji, Muramoto

AU - Hisao, Kamiya

AU - Tadayoshi, Shiba

N1 - Funding Information: We thank C. Segawa, R. Toyonaga, S. Tsuto, T. Uematsua nd A. Yamashitaf or excellentt echnicala ssis-tance.T his work was supportedi n part by grants-in-aid from the Ministry of Education,S ciencea nd Culture of Japan.

PY - 1993/6/30

Y1 - 1993/6/30

N2 - We have isolated cDNA clones coding for a lectin (BRA-3) from the acorn barnacle, Megabalanus rosa. Sequence comparison of the cDNA clones has revealed polymorphism in the BRA-3 mRNA, which results from single-nucleotide (nt) differences at three positions. All three differences are within the coding region and cause conservative amino acid (aa) changes. The BRA-3 gene is composed of four exons, and the three single-nt differences are located on different exons. In addition, the BRA-3 mRNA and BRA-3 protein levels increased during early summer in a similar fashion, indicating that BRA-3 production is regulated mainly at the level of transcription.

AB - We have isolated cDNA clones coding for a lectin (BRA-3) from the acorn barnacle, Megabalanus rosa. Sequence comparison of the cDNA clones has revealed polymorphism in the BRA-3 mRNA, which results from single-nucleotide (nt) differences at three positions. All three differences are within the coding region and cause conservative amino acid (aa) changes. The BRA-3 gene is composed of four exons, and the three single-nt differences are located on different exons. In addition, the BRA-3 mRNA and BRA-3 protein levels increased during early summer in a similar fashion, indicating that BRA-3 production is regulated mainly at the level of transcription.

KW - Carbohydrate recognition domain

KW - exon/intron structure

KW - gene expression

KW - marine invertebrate

KW - polymerase chain reaction

KW - polymorphism

KW - recombinant DNA

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DO - 10.1016/0378-1119(93)90570-S

M3 - Article

C2 - 8514190

AN - SCOPUS:0027280760

VL - 128

SP - 251

EP - 255

JO - Gene

JF - Gene

SN - 0378-1119

IS - 2

ER -

Acorn barnacle Megabalanus rosa lectin (BRA-3): cDNA cloning, gene structure and seasonal changes of mRNA and protein levels

Abstract

Keywords

ASJC Scopus subject areas

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