Acorn barnacle Megabalanus rosa lectin (BRA-3): cDNA cloning, gene structure and seasonal changes of mRNA and protein levels

Takamatsu Nobuhiko, Takeda Tadashi, Kojima Miho, Heishi Masayuki, Muramoto Koji, Kamiya Hisao, Shiba Tadayoshi

    Research output: Contribution to journalArticlepeer-review

    18 Citations (Scopus)

    Abstract

    We have isolated cDNA clones coding for a lectin (BRA-3) from the acorn barnacle, Megabalanus rosa. Sequence comparison of the cDNA clones has revealed polymorphism in the BRA-3 mRNA, which results from single-nucleotide (nt) differences at three positions. All three differences are within the coding region and cause conservative amino acid (aa) changes. The BRA-3 gene is composed of four exons, and the three single-nt differences are located on different exons. In addition, the BRA-3 mRNA and BRA-3 protein levels increased during early summer in a similar fashion, indicating that BRA-3 production is regulated mainly at the level of transcription.

    Original languageEnglish
    Pages (from-to)251-255
    Number of pages5
    JournalGene
    Volume128
    Issue number2
    DOIs
    Publication statusPublished - 1993 Jun 30

    Keywords

    • Carbohydrate recognition domain
    • exon/intron structure
    • gene expression
    • marine invertebrate
    • polymerase chain reaction
    • polymorphism
    • recombinant DNA

    ASJC Scopus subject areas

    • Genetics

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