Acidic vesicles in living hyphae of an arbuscular mycorrhizal fungus, Gigaspora margarita

Katsuharu Saito, Yukari Kuga-Uetake, Masanori Saito

Research output: Contribution to journalArticlepeer-review

18 Citations (Scopus)

Abstract

Localization and movement of organelles in living hyphae of an arbuscular mycorrhizal fungus, Gigaspora margarita, were observed using a combination of fluorescent probes and laser-scanning confocal microscopy. Dense, evenly distributed acidic vesicles were visible in germ tubes and extraradical hyphae using DIC with the fluorescent acidotropic probe LysoTracker. These vesicles were distinct from both tubular vacuoles stained with DFFDA and lipid bodies stained with BODIPY 493/503 or Nile Red. Tubular vacuole bundles appeared to be influenced by the bidirectional cytoplasmic streaming of acidic vesicles and lipid bodies. Movement of the acidic vesicles occurred bidrectionally at different rates. The size and distribution of lipid bodies were variable. Based on our observations, the function of these organelles is discussed in relation to nutrient translocation in arbuscular mycorrhizas.

Original languageEnglish
Pages (from-to)231-237
Number of pages7
JournalPlant and Soil
Volume261
Issue number1-2
DOIs
Publication statusPublished - 2004 Apr 1

Keywords

  • BODIPY 493/503
  • Gigaspora margarita
  • LysoTracker Red
  • Oregon Green 488 carboxy-DFFDA
  • arbuscular mycorrhizal fungi
  • laser-scanning confocal microscopy

ASJC Scopus subject areas

  • Soil Science
  • Plant Science

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