Acidic C-tail of HMGB1 is required for its target binding to nucleosome linker DNA and transcription stimulation

Tetsuya Ueda, Hiroyasu Chou, Toshifumi Kawase, Hitoshi Shirakawa, Michiteru Yoshida

Research output: Contribution to journalArticlepeer-review

64 Citations (Scopus)

Abstract

HMGB1, a nonhistone chromosomal protein in higher eukaryotic nuclei, consists of two DNA binding motifs called HMG boxes and an acidic C-tail comprising a continuous array of 30 acidic amino acid residues. In the preceding study, we showed that the acidic C-tail of HMGB1 is required for transcription stimulation accompanied by chromatin decondensation in cultured cells. However, details of the involvement of the acidic C-tail in transcription stimulation were not clear. To clarify the mechanism of transcription stimulation by the acidic C-tail, we assessed the effect of the acidic C-tail on the transcription stimulation and nucleosome binding. Transcription stimulation assays using acidic C-tail deletion mutants showed that the five amino acid residues at the C-terminal end of HMGB1, a DDDDE sequence, are essential for the stimulation. The DDDDE sequence was also required for the preferential binding of HMGB1 to nucleosome linker DNA, which is a cognate HMGB1 binding site in chromatin. Cross-linking and far-Western experiments demonstrated that the DDDDE sequence interacts with the core histone H3 N-tail. These results strongly suggest that the interaction between the DDDDE sequence of HMGB1 and the H3 N-tail is a key factor for the transcription stimulation by HMGB1 as well as the preferential binding of HMGB1 to chromatin.

Original languageEnglish
Pages (from-to)9901-9908
Number of pages8
JournalBiochemistry
Volume43
Issue number30
DOIs
Publication statusPublished - 2004 Aug 3
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry

Fingerprint Dive into the research topics of 'Acidic C-tail of HMGB1 is required for its target binding to nucleosome linker DNA and transcription stimulation'. Together they form a unique fingerprint.

Cite this