Acidic amino acid transport characteristics of a newly developed conditionally immortalized rat type 2 astrocyte cell line (TR-AST)

K. Tetsuka, K. I. Hosoya, S. Ohtsuki, H. Takanaga, N. Yanai, M. Ueda, M. Obinata, T. Terasaki

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

To characterize acidic amino acid transport in type 2 astrocytes, we established conditionally immortalized rat astrocyte cell lines (TR-AST) from newly developed transgenic rats harboring temperature-sensitive SV40 large T-antigen gene. TR-AST exhibited positive immunostaining for anti-GFAP antibody and A2B5 antibody, characteristics associated with type 2 astrocytes, and expressed glutamine synthetase. Acidic amino acid transporters, GLT-1 and system xc-, which consists of xCT and 4F2hc, were expressed in all TR-ASTs by RT-PCR. On the other hand, GLAST expression was found in TR-AST3 and 5. The characteristics of [3H]L-glutamic acid (L-Glu) uptake by TR-AST5 include an Na+-dependent and Na+-independent manner, concentration-dependence, and inhibition by L-aspartic acid (L-Asp) and D-aspartic acid (D-Asp). The corresponding Michaelis-Menten constants for the Na+-dependent and Na+-independent process were 36.3 μM and 155 μM, respectively. [3H]L-Asp and [3H]D-Asp uptake by TR-AST5 had an Na+-dependent and Na+-independent manner. This study demonstrated that GLT-1, system xc-, and GLAST were expressed in TR-AST, which has the characteristics of type 2 astrocytes and is able to transport acidic amino acids.

Original languageEnglish
Pages (from-to)197-203
Number of pages7
JournalCell structure and function
Volume26
Issue number4
DOIs
Publication statusPublished - 2001 Nov 21

Keywords

  • Acidic amino acid
  • GLAST
  • GLT-1
  • System x
  • Transport
  • Type 2 astorcyte

ASJC Scopus subject areas

  • Physiology
  • Molecular Biology
  • Cell Biology

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    Tetsuka, K., Hosoya, K. I., Ohtsuki, S., Takanaga, H., Yanai, N., Ueda, M., Obinata, M., & Terasaki, T. (2001). Acidic amino acid transport characteristics of a newly developed conditionally immortalized rat type 2 astrocyte cell line (TR-AST). Cell structure and function, 26(4), 197-203. https://doi.org/10.1247/csf.26.197