Acid hydrolysis of protein in a microcapillary tube for the recovery of tryptophan

Abayomi Peter Adebiyi, Dong Hao Jin, Tomohisa Ogawa, Koji Muramoto

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)

Abstract

The acid hydrolysis of proteins was miniaturized and simplified by employing microcapillary tubes (100 μl in volume) with 6 M HCl containing 1% 2-mercaptoethanol and 3% phenol for an amino acid compositional analysis. The method not only eliminated the laborious evacuation step for the hydrolysis tube but also decreased the destruction of tryptophan during hydrolysis. The recovery of tryptophan was 79% by acid hydrolysis at 145°C for 4 h. Since the acid mixture could be removed under vacuum, the hydrolysate was subjected to an amino acid analysis without neutralization or dilution.

Original languageEnglish
Pages (from-to)255-257
Number of pages3
JournalBioscience, Biotechnology and Biochemistry
Volume69
Issue number1
DOIs
Publication statusPublished - 2005 Jan

Keywords

  • Amino acid analysis
  • Hydrolyis
  • Protein
  • Tryptophan

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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