Acid hydrolysis of protein in a microcapillary tube for the recovery of tryptophan

Abayomi Peter Adebiyi, Dong Hao Jin, Tomohisa Ogawa, Koji Muramoto

    Research output: Contribution to journalArticlepeer-review

    15 Citations (Scopus)


    The acid hydrolysis of proteins was miniaturized and simplified by employing microcapillary tubes (100 μl in volume) with 6 M HCl containing 1% 2-mercaptoethanol and 3% phenol for an amino acid compositional analysis. The method not only eliminated the laborious evacuation step for the hydrolysis tube but also decreased the destruction of tryptophan during hydrolysis. The recovery of tryptophan was 79% by acid hydrolysis at 145°C for 4 h. Since the acid mixture could be removed under vacuum, the hydrolysate was subjected to an amino acid analysis without neutralization or dilution.

    Original languageEnglish
    Pages (from-to)255-257
    Number of pages3
    JournalBioscience, Biotechnology and Biochemistry
    Issue number1
    Publication statusPublished - 2005 Jan


    • Amino acid analysis
    • Hydrolyis
    • Protein
    • Tryptophan

    ASJC Scopus subject areas

    • Biotechnology
    • Analytical Chemistry
    • Biochemistry
    • Applied Microbiology and Biotechnology
    • Molecular Biology
    • Organic Chemistry


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