Acellular and cellular hemoglobin solutions as vasoconstrictive factor

K. Nakai, N. Matsuda, S. Sekiguchi, M. Amano, T. Ohta, S. Tokuyama, K. Akama, E. Tsuchida

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)

Abstract

The inhibitory effects of acellular and cellular hemoglobin (Hb) solutions on endothelium-dependent vasorelaxation were investigated in rabbit thoracic aortic strips. As acellular Hb solutions, 2,3-diphosphoglycerate (DPG)-depleted Hb and pyridoxylated Hb were examined. Cellular Hb solutions included washed human fresh red cells and liposome Hb encapsulated with pyridoxal-5'-phosphate (PLP). The tissues were precontracted with phenylephrine (PE), after which acetylcholine (ACh) was added to elicit a steady-state relaxation. Acellular Hb solutions cumulatively reversed ACh-induced relaxation, and these inhibitory effects reached a plateau at 10 μg/ml. Increasing oxygen affinity by pyridoxylation had little effect on this. In contrast, both red cells and liposome Hb solution showed moderate inhibitory effects, and they reached a plateau at 1 mg/ml. These findings indicate that acellular Hb solutions are more potent inhibitors than cellular Hb solutions by a factor of about 100, and that the encapsulation of Hb is a preferable method to mimic the red cell.

Original languageEnglish
Pages (from-to)559-564
Number of pages6
JournalArtificial Cells, Blood Substitutes, and Biotechnology
Volume22
Issue number3
DOIs
Publication statusPublished - 1994 Jan
Externally publishedYes

ASJC Scopus subject areas

  • Biotechnology
  • Biomedical Engineering

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