Accumulation of raffinose in rice seedlings overexpressing OsWRKY11 in relation to desiccation tolerance

Xiaolan Wu, Sachie Kishitani, Yukihiro Ito, Kinya Toriyama

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

We previously reported that transgenic rice (Oryza sativa L.) lines overexpressing OsWRKY11 showed significant desiccation tolerance, as indicated by their slower water loss in detached leaves. Here we examined the contents of sucrose, glucose, fructose and raffinose. Raffinose was shown to accumulate at a significantly higher level in the transgenic plants overexpressing OsWRKY11. Microarray analysis of gene expression profile indicated that the gene expression of Os07g0209100 encoding raffinose synthase and that of Os07g0687900 encoding galactinol synthase were upregulated. These results suggest that OsWRKY11 induced activation of these genes involved in raffinose synthesis and the accumulated raffinose played an important role in the desiccation tolerance of the OsWRKY11-overexpressed plants.

Original languageEnglish
Pages (from-to)431-434
Number of pages4
JournalPlant Biotechnology
Volume26
Issue number4
DOIs
Publication statusPublished - 2009 Jan 1

Keywords

  • Desiccation tolerance
  • Raffinose
  • Transgenic rice
  • WRKY

ASJC Scopus subject areas

  • Biotechnology
  • Agronomy and Crop Science
  • Plant Science

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