Accumulation of gamma/delta T cells in the lungs and their roles in neutrophil-mediated host defense against pneumococcal infection

Chikara Nakasone, Natsuo Yamamoto, Masashi Nakamatsu, Takeshi Kinjo, Kazuya Miyagi, Kaori Uezu, Kiwamu Nakamura, Futoshi Higa, Hiromichi Ishikawa, Rebecca L. O'Brien, Koichi Ikuta, Mitsuo Kaku, Jiro Fujita, Kazuyoshi Kawakami

Research output: Contribution to journalArticlepeer-review

57 Citations (Scopus)

Abstract

The present study was designed to elucidate the role of Vγ4+ γδ T cells, a major subset of pulmonary γδ T cells, in host defense against infection with Streptococcus pneumoniae. The proportion and number of whole γδ T cells, identified as CD3+ and TCR-δ+ cells, and Vγ4+ γδ T cells, identified as CD3+ and TCR-Vγ4+ cells, increased in the lungs at 3, 6 and 12 h post-infection. Survival of infected mice and lung bacterial clearance were severely impaired in TCR-Vγ4-/- mice compared with control wild-type (WT) mice. The impaired host protection in TCR-Vγ4-/- mice correlated well with attenuated recruitment of neutrophils in lungs. MIP-2 and TNF-α synthesis in the infected tissues was significantly reduced in TCR-Vγ4-/- mice compared with WT mice. Similar results were noted in the synthesis of TNF-α, but not clearly of MIP-2, by lung leukocytes stimulated with live bacteria. Our results demonstrate that Vγ4+ γδ T cells play an important role in the neutrophil-mediated host defense against S. pneumoniae infection by promoting the synthesis of TNF-α and possibly of MIP-2 in the lungs.

Original languageEnglish
Pages (from-to)251-258
Number of pages8
JournalMicrobes and Infection
Volume9
Issue number3
DOIs
Publication statusPublished - 2007 Mar

Keywords

  • Gamma/delta T cells
  • Host defense
  • MIP-2
  • Neutrophils
  • Pneumococcal infection
  • TNF-α

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Infectious Diseases

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