Accumulation of chloroplast DNA sequences on the Y chromosome of Silene latifolia

Eduard Kejnovsky, Zdenek Kubat, Roman Hobza, Martina Lengerova, Shusei Sato, Satoshi Tabata, Kiichi Fukui, Sachihiro Matsunaga, Boris Vyskot

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

Silene latifolia is a model dioecious plant with heteromorphic sex chromosomes. The Y chromosome is the largest in this species. Theoretical models propose an accumulation of repetitive DNA sequences in non-recombining parts of the Y chromosome. In this study, we isolated a BAC7H5 clone preferentially hybridizing to the Y chromosome of S. latifolia. Sequence analysis revealed that this BAC7H5 contains part of the chloroplast genome, indicating that these chloroplast sequences have accumulated on the Y chromosome and also may contribute to its large size. We constructed Y chromosome- and X chromosome-specific libraries and screened them to find Y- and/or X-linked copies of chloroplast sequences. Sequence analysis revealed higher divergence of a non-genic region of the chloroplast sequences located on the Y chromosome while genic regions tested showed only very low (max 0.9%) divergence from their chloroplast homologues.

Original languageEnglish
Pages (from-to)167-175
Number of pages9
JournalGenetica
Volume128
Issue number1-3
DOIs
Publication statusPublished - 2006 Sep

Keywords

  • Accumulation
  • Chloroplast DNA
  • Silene latifolia
  • Y chromosome

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Genetics
  • Plant Science
  • Insect Science

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    Kejnovsky, E., Kubat, Z., Hobza, R., Lengerova, M., Sato, S., Tabata, S., Fukui, K., Matsunaga, S., & Vyskot, B. (2006). Accumulation of chloroplast DNA sequences on the Y chromosome of Silene latifolia. Genetica, 128(1-3), 167-175. https://doi.org/10.1007/s10709-005-5701-0