Accumulation of beta-amyloid in the brain microvessels accompanies increased hyperphosphorylated tau proteins following microsphere embolism in aged rats

F. Han, A. Ali Raie, N. Shioda, Z. H. Qin, K. Fukunaga

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

To define mechanisms underlying neurovascular injury following brain embolism-induced neurodegeneration, we investigated temporal and spatial pathological changes in brain microvessels up to 12 weeks after microsphere embolism (ME) induction in aged male rats. Mild ME upregulated endothelial nitric oxide synthase (eNOS) and protein tyrosine nitration in brain microvessels. Strong β-amyloid immunoreactivity coincident with increased eNOS immunoreactivity was observed in microvessels. Immunoblotting of purified brain microvessels revealed that β-amyloid accumulation significantly increased 1 week after ME induction and remained elevated for 12 weeks. Importantly, β-amyloid accumulation in brain parenchyma was also observed in areas surrounding injured microvessels at 12 weeks. Levels of Alzheimer's-related hyperphosphorylated tau proteins also concomitantly increased in neurons surrounding regions of β-amyloid accumulation 12 weeks after ME induction, as did glycogen synthase kinase (GSK3β) (Tyr-216) phosphorylation. Taken together, ME-induced aberrant eNOS expression and subsequent protein tyrosine nitration in microvessels preceded β-amyloid accumulation both in microvessels and brain parenchyma, leading to hyperphosphorylation of neuronal tau proteins through GSK3β activation.

Original languageEnglish
Pages (from-to)414-427
Number of pages14
JournalNeuroscience
Volume153
Issue number2
DOIs
Publication statusPublished - 2008 May 2

Keywords

  • Alzheimer's disease
  • beta-amyloid
  • eNOS
  • microvascular
  • peroxynitrite
  • tau

ASJC Scopus subject areas

  • Neuroscience(all)

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