Accessory cell function of a human colonic epithelial cell line HT-29 for bacterial superantigens

Z. X. Liu, S. Sugawara, N. Hiwatashi, M. Noguchi, H. Rikiishi, K. Kumagai, T. Toyota

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The expression and up-regulation of cell adhesion molecules on a human colonic epithelial cell line HT-29, and the peripheral blood T lymphocyte proliferation responses to bacterial superantigens presented by this cell line were investigated, compared with peripheral blood monocytes. In HT-29 cells, there was constitutive expression of intercellular adhesion molecule- 1 (ICAM-1) and lymphocyte function associated antigen-3 (LFA-3) at a low level, but no constitutive expression of HLA-DR, LFA-1 B7-1 anti B7-2 molecules. After stimulation with the supernatants of staphylococcal enterotoxin B (SEB)-stimulated peripheral blood mononuclear cells for 48 h, there was significant up-regulation of HLA-DR and ICAM-1 molecules (both >90% positive). However, this stimulation had no effect on the expression of LFA-1, B7-1, B7-2 and LFA-3 molecules. In the presence of all tested superantigens SEB, toxic shock syndrome toxin-1, and streptococcal pyogenic exotoxin A, stimulated HT-29 cells caused significant T cell proliferation. When monocytes were used as antigen-presenting cells (APC), the MoAbs against HLA-DR, B7-2 and LFA-3 showed a significant inhibition of SEB- induced T cell proliferation. Anti-ICAM-1 MoAb had no effect on this response. On the other hand, when stimulated HT-29 cells were used as APC, the MoAbs against HLA-DR and ICAM-1 significantly inhibited SEB-induced T cell proliferation. In contrast to monocytes, anti B7-2 and anti-LFA-3 had no effect on this response. SEB could not induce HT-29 cells to produce IL- 8 directly; however, SEB significantly induced the stimulated HT-29 cells to produce IL-8 in the presence of T cells. Thus these data demonstrate that the products of superantigen-stimulated T cell activation can increase the expression of HLA-DR and ICAM-1 molecules on HT-29 cells significantly. Stimulated HT-29 cells can serve as APC to bacterial superantigens. This response is an HLA-DR and ICAM-1-dependent, but B7-2- and LFA-3-independent process, which was different from professional APC monocytes.

Original languageEnglish
Pages (from-to)384-391
Number of pages8
JournalClinical and Experimental Immunology
Volume108
Issue number3
DOIs
Publication statusPublished - 1997

Keywords

  • IL-8
  • antigen presentation
  • costimulatory
  • intestinal epithelial cell
  • molecule
  • superantigen

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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