@article{c77843b29aaa4dcaab3b7ce8bfee2568,
title = "Acceptor range of endo-β-n-acetylglucosaminidase mutant endo-CC N180H: From monosaccharide to antibody",
abstract = "The endo-β-N-acetylglucosaminidase mutant endo-CC N180H transfers glycan from sialylglycopeptide (SGP) to various acceptors. The scope and limitations of low-molecular-weight acceptors were investigated. Several homogeneous glycan-containing compounds, especially those with potentially useful labels or functional moieties, and possible reagents in glycoscience were synthesized. The 1,3-diol structure is important in acceptor molecules in glycan transfer reactions mediated by endo-CC N180H as well as by endo-M-N175Q. Glycan remodelling of antibodies was explored using core-fucose-deficient anti-CCR4 antibody with SGP and endo-CC N180H. Homogeneity of the glycan in the antibody was confirmed by mass spectrometry without glycan cleavage.",
keywords = "Antibody, Endo-CC N180H, Endo-β-N-acetylglucosaminidase mutant, Glycan transfer, Sialylglycopeptide",
author = "Shino Manabe and Yoshiki Yamaguchi and Junpei Abe and Kana Matsumoto and Yukishige Ito",
note = "Funding Information: Data accessibility. Electronic supplementary material including acceptor preparation, 1Hand 13C-NMR spectra, and time-course of glycan transfer reaction by endo-CC N180H is available. Authors{\textquoteright} contributions. S.M. and Y.Y. conceived, designed the experiments and wrote the manuscript. S.M. synthesized low-molecular-weight acceptors and characterized compounds. J.A. carried out the glycan transfer reaction, and collected NMR spectra of products. K.M. carried out antibody-glycan remodelling. Y.I. contributed to the discussion of the results. Competing interests. We declare we have no competing interests. Funding. S.M. was supported by a MEXT/JSPS KAKENHI (grant no. 24590041) and Japan Agency of Medical Research and Development (17cm0106220h0002). Acknowledgements. We thank Ms Akemi Takahashi for her technical assistance. We thank Dr Kaori Otsuki, Dr Masaya Usui and Dr Aya Abe in the Research Resource Center at the Brain Science Center, RIKEN for MS measurements. We thank Dr Takemichi Nakamura in RIKEN Center for Sustainable Resource Science for HRMS measurements. We also thank Fushimi Pharmaceutical Co. Ltd for supplying the endo-CC N180H and SGP. Funding Information: S.M. was supported by a MEXT/JSPS KAKENHI (grant no. 24590041) and Japan Agency of Medical Research and Development (17cm0106220h0002). Acknowledgements. We thank Ms Akemi Takahashi for her technical assistance. We thank Dr Kaori Otsuki, Dr Masaya Usui and Dr Aya Abe in the Research Resource Center at the Brain Science Center, RIKEN for MS measurements. We thank Dr Takemichi Nakamura in RIKEN Center for Sustainable Resource Science for HRMS measurements. We also thank Fushimi Pharmaceutical Co. Ltd for supplying the endo-CC N180H and SGP. Publisher Copyright: {\textcopyright} 2018 The Authors.",
year = "2018",
month = may,
day = "16",
doi = "10.1098/rsos.171521",
language = "English",
volume = "5",
journal = "Royal Society Open Science",
issn = "2054-5703",
publisher = "The Royal Society",
number = "5",
}