TY - JOUR
T1 - Acceleration of UVB-induced photoageing in nrf2 gene-deficient mice
AU - Hirota, Ayako
AU - Kawachi, Yasuhiro
AU - Yamamoto, Masayuki
AU - Koga, Tsutomu
AU - Hamada, Kazuhiko
AU - Otsuka, Fujio
PY - 2011/8
Y1 - 2011/8
N2 - Ultraviolet (UV) radiation is one of the most important environmental factors involved in the pathogenesis of premature skin ageing, termed photoageing. The harmful effects of UV in photoageing are associated with the generation of reactive oxygen species, and cellular antioxidants act to prevent the occurrence and reduce the severity of UV-induced photoageing. The transcription factor Nrf2 and its cytoplasmic anchor protein, Keap1, are central regulators of the cellular antioxidant response. Here, we investigated the role of the Nrf2-Keap1 pathway in photoageing using nrf2 gene-deficient (nrf2 -/-) mice. Our results indicated that UVB-irradiated nrf2 -/- mice showed accelerated photoageing, such as coarse wrinkle formation, loss of skin flexibility, epidermal thickening and deposition of extracellular matrix in the upper dermis. In addition, nrf2 -/- mice also showed an increase in cutaneous reactivity for the lipid peroxidation product 4-hydroxy-2-nonenal and a significant decrease in cutaneous glutathione level. These findings indicate that Nrf2 plays the important role in the protection against UVB-induced photoageing.
AB - Ultraviolet (UV) radiation is one of the most important environmental factors involved in the pathogenesis of premature skin ageing, termed photoageing. The harmful effects of UV in photoageing are associated with the generation of reactive oxygen species, and cellular antioxidants act to prevent the occurrence and reduce the severity of UV-induced photoageing. The transcription factor Nrf2 and its cytoplasmic anchor protein, Keap1, are central regulators of the cellular antioxidant response. Here, we investigated the role of the Nrf2-Keap1 pathway in photoageing using nrf2 gene-deficient (nrf2 -/-) mice. Our results indicated that UVB-irradiated nrf2 -/- mice showed accelerated photoageing, such as coarse wrinkle formation, loss of skin flexibility, epidermal thickening and deposition of extracellular matrix in the upper dermis. In addition, nrf2 -/- mice also showed an increase in cutaneous reactivity for the lipid peroxidation product 4-hydroxy-2-nonenal and a significant decrease in cutaneous glutathione level. These findings indicate that Nrf2 plays the important role in the protection against UVB-induced photoageing.
KW - Nrf2
KW - Photoageing
KW - Reactive oxygen species
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U2 - 10.1111/j.1600-0625.2011.01292.x
DO - 10.1111/j.1600-0625.2011.01292.x
M3 - Article
C2 - 21569103
AN - SCOPUS:79960311040
VL - 20
SP - 664
EP - 668
JO - Experimental Dermatology
JF - Experimental Dermatology
SN - 0906-6705
IS - 8
ER -