TY - JOUR
T1 - Accelerated neutrophil apoptosis in mice lacking A1-a, a subtype of the bcl-2-related A1 gene
AU - Hamasaki, Azumi
AU - Sendo, Fujiro
AU - Nakayama, Keiko
AU - Ishida, Noriko
AU - Negishi, Izumi
AU - Nakayama, Kei Ichi
AU - Hatakeyama, Shigetsugu
PY - 1998/12/7
Y1 - 1998/12/7
N2 - To elucidate the role of A1, a new member of the Bcl-2 family of apoptosis regulators active in hematopoietic cell apoptosis, we established mice lacking A1-a, a subtype of the A1 gene in mice (Al-a(-/-) mice). Spontaneous apoptosis of peripheral blood neutrophils of A1-a(-/-) mice was enhanced compared with that of either wild-type mice or heterozygous mutants (A1-a(+/-) mice). Neutrophil apoptosis inhibition induced by lipopolysaccharide treatment in vitro or transendothelial migration in vivo observed in wild-type mice was abolished in both A1-a(-/-) and A1-a(+/-) animals. On the other hand, the extent of tumor necrosis factor α-induced acceleration of neutrophil apoptosis did not differ among A1-a(-/-), A1- a(+/-), and wild-type mice. The descending order oral mRNA expression was wild-type, A1-a(+/-), and A1-a(-/-). Taken together, these results suggest that A1 is involved in inhibition of certain types of neutrophil apoptosis.
AB - To elucidate the role of A1, a new member of the Bcl-2 family of apoptosis regulators active in hematopoietic cell apoptosis, we established mice lacking A1-a, a subtype of the A1 gene in mice (Al-a(-/-) mice). Spontaneous apoptosis of peripheral blood neutrophils of A1-a(-/-) mice was enhanced compared with that of either wild-type mice or heterozygous mutants (A1-a(+/-) mice). Neutrophil apoptosis inhibition induced by lipopolysaccharide treatment in vitro or transendothelial migration in vivo observed in wild-type mice was abolished in both A1-a(-/-) and A1-a(+/-) animals. On the other hand, the extent of tumor necrosis factor α-induced acceleration of neutrophil apoptosis did not differ among A1-a(-/-), A1- a(+/-), and wild-type mice. The descending order oral mRNA expression was wild-type, A1-a(+/-), and A1-a(-/-). Taken together, these results suggest that A1 is involved in inhibition of certain types of neutrophil apoptosis.
KW - A1
KW - Apoptosis
KW - Gene disruption
KW - Neutrophil
KW - bcl-2-related gene
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U2 - 10.1084/jem.188.11.1985
DO - 10.1084/jem.188.11.1985
M3 - Article
C2 - 9841913
AN - SCOPUS:0031743756
VL - 188
SP - 1985
EP - 1992
JO - Journal of Experimental Medicine
JF - Journal of Experimental Medicine
SN - 0022-1007
IS - 11
ER -