Accelerated evolution of crotalinae snake venom gland serine proteases

Masanobu Deshimaru, Tomohisa Ogawa, Kin ichi Nakashima, Ikuo Nobuhisa, Takahito Chijiwa, Yasuyuki Shimohigashi, Yasuyuki Fukumaki, Mineo Niwa, Ikuo Yamashina, Shosaku Hattori, Motonori Ohno

Research output: Contribution to journalArticlepeer-review

150 Citations (Scopus)

Abstract

Eight cDNAs encoding serine proteases isolated from Trimeresurus flavoviridis (habu snake) and T. gramineus (green habu snake) venom gland cDNA libraries showed that nonsynonymous nucleotide substitutions have accumulated in the mature protein-coding regions to cause amino acid changes. Southern blot analysis of T. flavoviridis genomic DNAs using two proper probes indicated that venom gland serine protease genes form a multigene family in the genome. These observations suggest that venom gland serine proteases have diversified their amino acid sequences in an accelerating manner. Since a similar feature has been previously discovered in crotalinae snake venom gland phospholipase A2 (PLA2) isozyme genes, accelerated evolution appears to be universal in plural isozyme families of crotalinae snake venom gland.

Original languageEnglish
Pages (from-to)83-88
Number of pages6
JournalFEBS Letters
Volume397
Issue number1
DOIs
Publication statusPublished - 1996 Nov 11
Externally publishedYes

Keywords

  • Evolution
  • Serine protease family
  • Snake venom
  • cDNA cloning

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Fingerprint

Dive into the research topics of 'Accelerated evolution of crotalinae snake venom gland serine proteases'. Together they form a unique fingerprint.

Cite this