Accelerated evolution in the protein-coding region of galectin cdnas, congerin I and congerin II, from skin mucus of conger eel (conger myriaster)

Tomohisa Ogawa, Chihiro Ishii, Daiji Kagawa, Koji Muramoto, Hisao Kamiya

Research output: Contribution to journalArticlepeer-review

38 Citations (Scopus)

Abstract

Two cDNAs encoding galectins named congerins I and II from the skin mucus of conger eel (Conger myriaster) were isolated and sequenced. Comparison of the nucleotide sequences of congerins I and II showed that the sequence similarities of the 5′ and 3′ untranslated regions (86 and 88%, respectively) were much higher than those of the protein-coding region (73%). The numbers of nucleotide substitutions per site (KN) for the untranslated regions are smaller than the numbers of nucleotide substitutions per synonymous site (KS) for the protein coding region. Furthermore, nonsynonymous nucleotide substitutions have accelerated more frequently than synonymous nucleotide substitutions in the protein coding region (KA/KS=2.57). These results suggest that accelerated substitutions have occurred in the protein-coding regions of galectin genes to generate diverse galectins with different molecular properties. Northern blot analysis showed that both congerins were expressed not only in the skin tissues but also in the stomach of conger eel.

Original languageEnglish
Pages (from-to)1203-1208
Number of pages6
JournalBioscience, Biotechnology and Biochemistry
Volume63
Issue number7
DOIs
Publication statusPublished - 1999 Jan 1

Keywords

  • Accelerated evolution
  • Conger eel
  • Congerin I and congerin II
  • Galectin
  • Skin mucus

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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