TY - JOUR
T1 - Absorption and effectiveness of orally administered low molecular weight collagen hydrolysate in rats
AU - Mari, Watanabe Kamiyama
AU - Muneshige, Shimizu
AU - Shin, Kamiyama
AU - Yasuki, Taguchi
AU - Hideyuki, Sone
AU - Fumiki, Morimatsu
AU - Hitoshi, Shirakawa
AU - Yuji, Furukawa
AU - Michio, Komai
PY - 2010/1/27
Y1 - 2010/1/27
N2 - Collagen, a major extracellular matrix macromolecule, is widely used for biomedical purposes. We investigated the absorption mechanism of low molecular weight collagen hydrolysate (LMW-CH) and its effects on osteoporosis in rats. When administered to Wistar rats with either [14C]proline (Pro group) or glycyl-[14C]prolyl-hydroxyproline (CTp group), LMW-CH rapidly increased plasma radioactivity. LMW-CH was absorbed into the blood of Wistar rats in the peptide form. Glycyl-prolylhydroxyproline tripeptide remained in the plasma and accumulated in the kidney. In both groups, radioactivity was retained at a high level in the skin until 14 days after administration. Additionally, the administration of LMW-CH to ovariectomized stroke-prone spontaneously hypertensive rats increased the organic substance content and decreased the water content of the left femur. Our findings show that LMW-CH exerts a beneficial effect on osteoporosis by increasing the organic substance content of bone.
AB - Collagen, a major extracellular matrix macromolecule, is widely used for biomedical purposes. We investigated the absorption mechanism of low molecular weight collagen hydrolysate (LMW-CH) and its effects on osteoporosis in rats. When administered to Wistar rats with either [14C]proline (Pro group) or glycyl-[14C]prolyl-hydroxyproline (CTp group), LMW-CH rapidly increased plasma radioactivity. LMW-CH was absorbed into the blood of Wistar rats in the peptide form. Glycyl-prolylhydroxyproline tripeptide remained in the plasma and accumulated in the kidney. In both groups, radioactivity was retained at a high level in the skin until 14 days after administration. Additionally, the administration of LMW-CH to ovariectomized stroke-prone spontaneously hypertensive rats increased the organic substance content and decreased the water content of the left femur. Our findings show that LMW-CH exerts a beneficial effect on osteoporosis by increasing the organic substance content of bone.
KW - Absorption; collagen tripeptide; low molecular weight collagen hydrolysate; osteoporosis; OVX; SHRSP
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U2 - 10.1021/jf9031487
DO - 10.1021/jf9031487
M3 - Article
C2 - 19957932
AN - SCOPUS:74849132803
SN - 0021-8561
VL - 58
SP - 835
EP - 841
JO - Journal of Agricultural and Food Chemistry
JF - Journal of Agricultural and Food Chemistry
IS - 2
ER -