ABP1 and ROP6 GTPase signaling regulate clathrin-mediated endocytosis in Arabidopsis roots

Xu Chen, Satoshi Naramoto, Stéphanie Robert, Ricardo Tejos, Christian Löfke, Deshu Lin, Zhenbiao Yang, Jiří Friml

    Research output: Contribution to journalArticlepeer-review

    120 Citations (Scopus)

    Abstract

    The dynamic spatial and temporal distribution of the crucial plant signaling molecule auxin is achieved by feedback coordination of auxin signaling and intercellular auxin transport pathways [1, 2]. Developmental roles of auxin have been attributed predominantly to its effect on transcription; however, an alternative pathway involving AUXIN BINDING PROTEIN1 (ABP1) has been proposed to regulate clathrin-mediated endocytosis in roots and Rho-like GTPase (ROP)-dependent pavement cell interdigitation in leaves [3, 4]. In this study, we show that ROP6 and its downstream effector RIC1 regulate clathrin association with the plasma membrane for clathrin-mediated endocytosis, as well as for its feedback regulation by auxin. Genetic analysis revealed that ROP6/RIC1 acts downstream of ABP1 to regulate endocytosis. This signaling circuit is also involved in the feedback regulation of PIN-FORMED 1 (PIN1) and PIN2 auxin transporters activity (via its constitutive endocytosis) and corresponding auxin transport-mediated processes, including root gravitropism and leave vascular tissue patterning. Our findings suggest that the signaling module auxin-ABP1-ROP6/RIC1-clathrin-PIN1/PIN2 is a shared component of the feedback regulation of auxin transport during both root and aerial development.

    Original languageEnglish
    Pages (from-to)1326-1332
    Number of pages7
    JournalCurrent Biology
    Volume22
    Issue number14
    DOIs
    Publication statusPublished - 2012 Jul 24

    ASJC Scopus subject areas

    • Biochemistry, Genetics and Molecular Biology(all)
    • Agricultural and Biological Sciences(all)

    Fingerprint Dive into the research topics of 'ABP1 and ROP6 GTPase signaling regulate clathrin-mediated endocytosis in Arabidopsis roots'. Together they form a unique fingerprint.

    Cite this