Abnormalities and the implication of retinoblastoma locus and its protein product in head and neck cancers

Junkichi Yokoyama, Kiyoto Shiga, Hironobu Sasano, Mamoru Suzuki, Tomonori Takasaka

Research output: Contribution to journalArticlepeer-review

21 Citations (Scopus)

Abstract

Abnormalities in the Retinoblastoma tumor suppressor gene (Rb) have been observed in a large number of human cancels. Loss of heterozygosity (LOH) is a common mode of allelic inactivation of Rb and other tumor suppressor genes. We investigated DNA from 45 primary human head and neck cancels to determine LOH at the Rb locus using a polymerase chain reaction-based restriction fragment length polymorphism assay. Of informative cases, we found LOH in 4 of 28 (14%) head and neck cancers. By immunohistochemical staining of Rb protein, we found that in one of ten LOH negative cases the nuclei of fibroblasts were stained with anti-Rb antibody but there was no nuclear staining tumor cells. These results suggest that inactivation of Rb protein is involved in the carcinogenesis of head and neck cancer at all levels of the process of protein expression: DNA, mRNA and protein.

Original languageEnglish
Pages (from-to)641-644
Number of pages4
JournalAnticancer research
Volume16
Issue number2
Publication statusPublished - 1996

Keywords

  • Head and neck cancer
  • Immunohistochemistry
  • LOH
  • Rb

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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