Abnormal cleavage is involved in the self-correction of bovine preimplantation embryos

Hiroki Nagai, Mai Okada, Yoko Nagai, Yoshiyuki Sakuraba, Hiroaki Okae, Ryosuke Suzuki, Satoshi Sugimura

Research output: Contribution to journalArticlepeer-review

Abstract

Chromosome instability leading to aneuploidy during early cleavage is well known in humans and cattle. Partial compaction (PC), which occurs only in some blastomeres, is suggested as a self-correction mechanism through which human embryos avoid aneuploid mosaicism. Partially compacted embryos show abnormal cleavages more frequently during early development; however, the mechanism by which blastomeres are excluded has not been elucidated. Here, we confirmed PC in approximately half of the tested bovine embryos, similar to that in human embryos. DNA sequencing of single-cell and intact embryos revealed that the morulae that excluded some blastomeres had euploidy, but many of the excluded blastomeres had aneuploidy. Time-lapse imaging of zygotes without the zona pellucida revealed that the excluded blastomeres underwent reverse and direct cleavages, which are abnormal cleavages, more frequently than the blastomeres involved in compaction. These results suggest the potential role of abnormal cleavage in the self-correction mechanism during the development of mammalian preimplantation embryos.

Original languageEnglish
Pages (from-to)76-82
Number of pages7
JournalBiochemical and biophysical research communications
Volume562
DOIs
Publication statusPublished - 2021 Jul 12

Keywords

  • Aneuploidy
  • Blastomere
  • Compaction
  • Self-correction
  • Time-lapse imaging

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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