Aberrant vegetative and reproductive development by overexpression and lethality by silencing of OsHAP3E in rice

Yukihiro Ito, Thiruvengadam Thirumurugan, Akiko Serizawa, Keiichiro Hiratsu, Masaru Ohme-Takagi, Nori Kurata

Research output: Contribution to journalArticlepeer-review

24 Citations (Scopus)

Abstract

We generated transgenic rice plants overexpressing OsHAP3E which encodes a subunit of a CCAAT-motif binding HAP complex. The OsHAP3E-overexpressing plants showed various abnormal morphologies both in their vegetative and reproductive phases. The OsHAP3E-overexpressing plants were dwarf with erected leaves and similar to brassinosteroid mutants in the vegetative phase. In the reproductive phase, dense panicle was developed, and occasionally successive generation of lateral rachises and formation of double flowers were observed. These phenotypes indicate association of OsHAP3E with determination of floral meristem identity. On the other hand, repression of OsHAP3E by RNAi or by overexpressing chimeric repressor fusion constructs brought about lethality to transformed cells, and almost no transformant was obtained. This suggests that the OsHAP3E function is essential for rice cells. Altogether, our loss-of-function and gain-of-function analyses suggest that OsHAP3E plays important pleiotropic roles in vegetative and reproductive development or basic cellular processes in rice.

Original languageEnglish
Pages (from-to)105-110
Number of pages6
JournalPlant Science
Volume181
Issue number2
DOIs
Publication statusPublished - 2011 Aug 1

Keywords

  • CCAAT-box
  • CRES-T
  • HAP3/NF-YB/CBF-A
  • Panicle
  • Rice

ASJC Scopus subject areas

  • Genetics
  • Agronomy and Crop Science
  • Plant Science

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