Aberrant Amygdala-Dependent Cued Fear Memory in Na + /Ca 2+ Exchanger 1 Heterozygous Mice

Shigeki Moriguchi, Satomi Kita, Ryo Inagaki, Yasushi Yabuki, Yuzuru Sasaki, Shun Ishikawa, Hiroyuki Sakagami, Takahiro Iwamoto, Kohji Fukunaga

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

Na + /Ca 2+ exchangers (NCXs) are mainly expressed in the plasma membrane and exchange one Ca 2+ for three Na + , depending on the electrochemical gradients across the plasma membrane. NCXs have three isoforms, NCX1–3, encoded by distinct genes in mammals. Here, we report that heterozygous mice lacking NCX1 (NCX1 +/− ) exhibit impaired amygdala-dependent cued fear memory. NCX1 +/− mice showed significant impairment in fear-related behaviors measured with the elevated-plus maze, light-dark, open-field, and marble-burying tasks. In addition, NCX1 +/− mice showed abnormality in cued fear memory but not in contextual fear memory in a fear-conditioning task. In immunohistochemical analyses, NCX1 +/− mice had significantly increased number of c-Fos-positive cells in the lateral amygdala (LA) but not in the central amygdala following fear-related tone stimuli. c-Fos expression peaked at 1 h. In concordance with the aberrant fear-related behaviors in NCX1 +/− mice, enhanced long-term potentiation was also observed in the LA of these mice. Furthermore, enhancement of CaMKII or CaMKIV activity in the LA was observed in NCX1 +/− mice by immunoblot analyses. In contrast, CaMKII +/− but not CaMKIV −/− mice insufficiently exhibited tone-induced cued fear memory and there was no increase in the number of c-Fos-positive cells in the LA. Altogether, the increased CaMKII activity and consequent c-Fos expression likely account for the dysregulation of amygdala-dependent cued fear memory in NCX1 +/− mice.

Original languageEnglish
Pages (from-to)4381-4394
Number of pages14
JournalMolecular Neurobiology
Volume56
Issue number6
DOIs
Publication statusPublished - 2019 Jun 1

Keywords

  • Amygdala-dependent cued fear memory
  • CaMKII
  • Lateral amygdala
  • Na /Ca exchanger
  • c-Fos expression

ASJC Scopus subject areas

  • Neurology
  • Cellular and Molecular Neuroscience

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