TY - JOUR
T1 - ABCB1 is upregulated in acquisition of taxane resistance
T2 - Lessons from esophageal squamous cell carcinoma cell lines
AU - Wang, Ruobing
AU - Sumarpo, Anton
AU - Saiki, Yuriko
AU - Chen, Na
AU - Sunamura, Makoto
AU - Horii, Akira
N1 - Funding Information:
We are grateful to Dr. B.L.S. Pierce (University of Maryland University College) for editorial work in the preparation of this manuscript and to Biomedical Research Core (Tohoku University School of Medicine) for technical support. This work was supported in part by Grant-in-Aid from JSPS KAKENHI Grant Numbers JP15K10084, 26460468, 26462074.
Publisher Copyright:
© 2016 Tohoku University Medical Press.
PY - 2016/12
Y1 - 2016/12
N2 - Esophageal cancer is one of the common malignancies worldwide, particularly in eastern African and Asian countries including Japan. Taxane (paclitaxel or docetaxel) is one of the effective chemotherapeutic reagents for patients with esophageal cancer, but acquisition of chemoresistance frequently occurs; this is one of the most frequent causes for therapeutic failure. In this study, we established three taxane resistant esophageal squamous cell carcinoma cell lines and explored possible mechanisms for the acquisition of chemoresistance. Microarray analyses indicated that the ABCB1 (ATP binding cassette subfamily B member 1) gene was significantly upregulated in taxane resistant esophageal cancer cell lines. Moreover, we found that siRNA mediated ABCB1 knockdown successfully restored drug sensitivity in both paclitaxel and docetaxel resistant esophageal cancer cell lines. In conclusion, we propose that ABCB1 might play a pivotal role in acquisition of taxane resistance and could be a promising target for treatment of patients with esophageal cancer after acquisition of taxane resistance.
AB - Esophageal cancer is one of the common malignancies worldwide, particularly in eastern African and Asian countries including Japan. Taxane (paclitaxel or docetaxel) is one of the effective chemotherapeutic reagents for patients with esophageal cancer, but acquisition of chemoresistance frequently occurs; this is one of the most frequent causes for therapeutic failure. In this study, we established three taxane resistant esophageal squamous cell carcinoma cell lines and explored possible mechanisms for the acquisition of chemoresistance. Microarray analyses indicated that the ABCB1 (ATP binding cassette subfamily B member 1) gene was significantly upregulated in taxane resistant esophageal cancer cell lines. Moreover, we found that siRNA mediated ABCB1 knockdown successfully restored drug sensitivity in both paclitaxel and docetaxel resistant esophageal cancer cell lines. In conclusion, we propose that ABCB1 might play a pivotal role in acquisition of taxane resistance and could be a promising target for treatment of patients with esophageal cancer after acquisition of taxane resistance.
KW - ABCB1
KW - Chemoresistance
KW - Docetaxel
KW - Esophageal squamous cell carcinoma
KW - Paclitaxel
UR - http://www.scopus.com/inward/record.url?scp=85006707318&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85006707318&partnerID=8YFLogxK
U2 - 10.1620/tjem.240.295
DO - 10.1620/tjem.240.295
M3 - Article
C2 - 27941276
AN - SCOPUS:85006707318
VL - 240
SP - 295
EP - 301
JO - Tohoku Journal of Experimental Medicine
JF - Tohoku Journal of Experimental Medicine
SN - 0040-8727
IS - 4
ER -
