A VSV-G pseudotyped HIV vector mediates efficient transduction of human pulmonary artery smooth muscle cells

Shou Lin Li, Xiao Yan Zhang, Hong Ling, Jun Ikeda, Kunio Shirato, Toshio Hattori

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

Attempts were made to infect human vascular smooth muscle cells derived from the pulmonary artery (hPASMC) with two different human immunodeficiency virus (HIV) vector systems. ADA/Luc or HXB2/Luc were generated by cotransfection of luciferase reporter gene vector, pNL4-3-Luc-E-R-, and one of two envelope expressing vectors, pSMADA (R5) or pSMHXB2 (X4). The VSV-G/Luc or VSV-G/GFP were produced by a three-plasmid expression system which consisted of vesicular stomatitis virus G protein (VSVG) expressing vector, packaging plasmid, and one of two reporter genes (pHR'-CMV-Luc or pHR'-CMV-GFP). We used hPASMC, U87.CD4.CCR5 and U87.CD4.CXCR4 for infection. Neither ADA/Luc nor HXB2/Luc could infect hPASMC, though they could infect U87.CD4 with corresponding coreceptors. On the other hand, the transduction of both VSV-G/Luc and VSV-G/GFP to hPASMC was remarkable. At day 3, the relative proportion of positive cells of hPASMC infected with VSV-G/GFP was 15%. The above finding indicates a direct role of HIV-1 infection in pulmonary hypertension 'a rare complication of HIV-1 infection' and HIV-based vectors could introduce foreign genes into hPASMC for gene therapy of pulmonary hypertension.

Original languageEnglish
Pages (from-to)1019-1025
Number of pages7
JournalMICROBIOLOGY and IMMUNOLOGY
Volume44
Issue number12
DOIs
Publication statusPublished - 2000 Dec

Keywords

  • HIV
  • Pulmonary hypertension
  • Smooth muscle cell
  • Vector

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Virology

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