A universal gelfoam 3-D histoculture method to establish patient-derived cancer cells (3D-PDCC) without fibroblasts from patient-derived xenografts

Jun Yamamoto, Norihiko Sugisawa, Kazuyuki Hamada, Hiroto Nishino, Kentaro Miyake, Ryusei Matsuyama, Sachiko Inubushi, Hirokazu Tanino, Michael Bouvet, Itaru Endo, Robert M. Hoffman

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Background/Aim: The direct placement of patient tumors in 2-D culture on plastic or glass surfaces has inhibited the establishment of patient-derived cancer cells (PDCCs). The aim of the present study was to develop universal and efficient methods to prepare PDCCs. Materials and Methods: Fragments of patient-derived xenograft (PDX) tumors established form colon cancer liver metastasis (1 mm3) were placed on Gelfoam and cultured in DMEM. Results: PDX tumor fragments were cultured on Gelfoam. Cancer cells migrated from the explant and formed distinct 3-D structures in the Gelfoam. Each of the three PDCCs showed a distinct morphology. The cultures were essentially all cancer cells without fibroblasts, the opposite of what usually occurs in 2-D culture on plastic or glass. Gelfoam cultures could be readily passaged from one Gelfoam cube to anothers suggesting indefinite culture potential. Conclusion: A potentially universal method to establish PDCC using PDX tumors and 3-D Gelfoam histoculture was developed.

Original languageEnglish
Pages (from-to)6765-6768
Number of pages4
JournalAnticancer research
Volume40
Issue number12
DOIs
Publication statusPublished - 2020 Dec
Externally publishedYes

Keywords

  • 3-D culture
  • Gelfoam
  • Histoculture
  • PDCC
  • PDX
  • Patient-derived cancer cells
  • Patient-derived xenograft

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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