A tubular biocontainer: Metal ion-induced 1D assembly of a molecularly engineered chaperonin

Shuvendu Biswas, Kazushi Kinbara, Nobuhiro Oya, Noriyuki Ishii, Hideki Taguchi, Takuzo Aida

Research output: Contribution to journalArticlepeer-review

71 Citations (Scopus)

Abstract

(Figure Presented) GroELSP/MC, prepared by genetic and chemical modifications of group I chaperonin protein GroEL, site-specifically possesses ∼28 photochromic (spiropyran [SP] and merocyanine [MC]) units in the entrance parts of its cavity. Addition of divalent metal ions such as Mg 2+ to a tris-HCl buffer solution of GroELSP/MC results in one-dimensional (1D) assembly of GroELSP/MC, affording cylindrical hollow fibers with a very large aspect ratio; the longest fiber was ∼2.5 μm long, corresponding to a 170-mer of GroELSP/MC (MW ≈ 1.4 × 108). When such long fibers are mixed with EDTA, they are cut into short-chain oligomers and eventually into monomeric GroELSP/MC. Similar to GroEL, GroELSP/MC possesses a large binding affinity toward denatured proteins. When GroELSP/MC undergoes 1D assembly after incubation with a denatured protein, guest-containing cylindrical fibers result.

Original languageEnglish
Pages (from-to)7556-7557
Number of pages2
JournalJournal of the American Chemical Society
Volume131
Issue number22
DOIs
Publication statusPublished - 2009 Jun 10

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

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