A Trk/HKT-type K+ transporter from Trypanosoma brucei

Marc Mosimann, Shinobu Goshima, Tanja Wenzler, Alexandra Lüscher, Nobuyuki Uozumi, Pascal Mäser

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13 Citations (Scopus)

Abstract

The molecular mechanisms of K+ homeostasis are only poorly understood for protozoan parasites. Trypanosoma brucei subsp. parasites, the causative agents of human sleeping sickness and nagana, are strictly extracellular and need to actively concentrate K+ from their hosts' body fluids. The T. brucei genome contains two putative K+ channel genes, yet the trypanosomes are insensitive to K+ antagonists and K+ channel-blocking agents, and they do not spontaneously depolarize in response to high extracellular K+ concentrations. However, the trypanosomes are extremely sensitive to K+ ionophores such as valinomycin. Surprisingly, T. brucei possesses a member of the Trk/HKT superfamily of monovalent cation permeases which so far had only been known from bacteria, archaea, fungi, and plants. The protein was named TbHKT1 and functions as a Na+ -independent K+ transporter when expressed in Escherichia coli, Saccharomyces cerevisiae, or Xenopus laevis oocytes. In trypanosomes, TbHKT1 is expressed in both the mammalian bloodstream stage and the Tsetse fly midgut stage; however, RNA interference (RNAi)-mediated silencing of TbHKT1 expression did not produce a growth phenotype in either stage. The presence of HKT genes in trypanosomatids adds a further piece to the enigmatic phylogeny of the Trk/HKT superfamily of K+ transporters. Parsimonial analysis suggests that the transporters were present in the first eukaryotes but subsequently lost in several of the major eukaryotic lineages, in at least four independent events.

Original languageEnglish
Pages (from-to)539-546
Number of pages8
JournalEukaryotic Cell
Volume9
Issue number4
DOIs
Publication statusPublished - 2010 Apr

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

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    Mosimann, M., Goshima, S., Wenzler, T., Lüscher, A., Uozumi, N., & Mäser, P. (2010). A Trk/HKT-type K+ transporter from Trypanosoma brucei. Eukaryotic Cell, 9(4), 539-546. https://doi.org/10.1128/EC.00314-09