A transfection reporter for the prevention of false-negative results in molecular beacon experiments

Tatsuya Toga, Isao Kuraoka, Akira Yasui, Shigenori Iwai

    Research output: Contribution to journalArticle

    3 Citations (Scopus)

    Abstract

    We previously developed a molecular beacon-type probe to detect the strand scission in cellular base excision repair and found that the phosphodiester linkages in the fluorophore/quencher linkers were cleaved. This reaction was applied to a transfection reporter, which contained the unmodified phosphodiester in the linker to another type of fluorophore. After cotransfection of cells with the probe and the reporter, the signals were used to detect the incision and to confirm the proper transfection, respectively. This method will contribute to the prevention of false-negative results in experiments using molecular beacon-type probes.

    Original languageEnglish
    Pages (from-to)9-11
    Number of pages3
    JournalAnalytical Biochemistry
    Volume440
    Issue number1
    DOIs
    Publication statusPublished - 2013 Jan 1

    Keywords

    • Base excision repair
    • Molecular beacon
    • Phosphorothioate
    • Thymine glycol

    ASJC Scopus subject areas

    • Biophysics
    • Biochemistry
    • Molecular Biology
    • Cell Biology

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