A transcriptional regulator, IscR, of Burkholderia multivorans acts as both repressor and activator for transcription of iron-sulfur cluster-biosynthetic isc operon

Shouta Nonoyama, Kouhei Kishida, Keiichiro Sakai, Yuji Nagata, Yoshiyuki Ohtsubo, Masataka Tsuda

Research output: Contribution to journalArticlepeer-review

Abstract

Bacterial iron-sulfur (Fe–S) clusters are essential cofactors for many metabolic pathways, and Fe–S cluster-containing proteins (Fe–S proteins) regulate the expression of various important genes. However, biosynthesis of such clusters has remained unknown in genus Burkholderia. Here, we clarified that Burkholderia multivorans ATCC 17616 relies on the ISC system for the biosynthesis of Fe–S clusters, and that the biosynthetic genes are organized as an isc operon, whose first gene encodes IscR, a transcriptional regulatory Fe–S protein. Transcription of the isc operon was repressed and activated under iron-rich and -limiting conditions, respectively, and Fur, an iron-responsive global transcriptional regulator, was indicated to indirectly regulate the expression of isc operon. Further analysis using a ΔiscR mutant in combination with a constitutive expression system of IscR and its derivatives indicated transcriptional repression and activation of isc operon by holo- and apo-forms of IscR, respectively, through their binding to the sequences within an isc promoter-containing (Pisc) fragment. Biochemical analysis using the Pisc fragment suggested that the apo-IscR binding sequence differs from the holo-IscR binding sequence. The results obtained in this study revealed a unique regulatory system for the expression of the ATCC 17616 isc operon that has not been observed in other genera.

Original languageEnglish
Pages (from-to)319-330
Number of pages12
JournalResearch in Microbiology
Volume171
Issue number8
DOIs
Publication statusPublished - 2020 Dec

Keywords

  • Burkholderia multivorans
  • Fe–S cluster
  • Iron
  • IscR
  • Transcriptional regulation

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

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