A trans-zeatin riboside in root xylem sap negatively regulates adventitious root formation on cucumber hypocotyls

T. Kuroha, H. Kato, T. Asami, S. Yoshida, H. Kamada, S. Satoh

Research output: Contribution to journalArticlepeer-review

58 Citations (Scopus)

Abstract

Shoot cultures of cucumber were used to analyse the roles of root-derived substances in adventitious root formation on hypocotyl tissues. Xylem sap collected from the roots of squash had a strong inhibitory effect on the formation of hypocotyl adventitious roots. Double-solvent extraction followed by fractionation with both normal and reverse phase column chromatographies and analysis by liquid chromatography/tandem mass spectrometry identified trans-zeatin riboside (ZR) as the primary suppressor of adventitious root formation. ZR was the predominant cytokinin present in the xylem sap, occurring at a concentration of 2×10-8 M. Application of ZR at concentrations from 3.16×10-9 M effected inhibition of adventitious root formation. These results suggest that ZR transported from roots via xylem sap may act as an endogenous suppressor of hypocotyl adventitious root formation in planta.

Original languageEnglish
Pages (from-to)2193-2200
Number of pages8
JournalJournal of experimental botany
Volume53
Issue number378
DOIs
Publication statusPublished - 2002 Nov 1
Externally publishedYes

Keywords

  • Adventitious root suppression
  • Cucumber
  • Cucumis sativus
  • Cucurbita
  • Cytokinin
  • Squash
  • Xylem sap
  • Zeatin riboside

ASJC Scopus subject areas

  • Physiology
  • Plant Science

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