A TLR7 agonist activates bovine Th1 response and exerts antiviral activity against bovine leukemia virus

Yamato Sajiki, Satoru Konnai, Tomohiro Okagawa, Naoya Maekawa, Hayato Nakamura, Yukinari Kato, Yasuhiko Suzuki, Shiro Murata, Kazuhiko Ohashi

Research output: Contribution to journalArticlepeer-review

Abstract

Bovine leukemia virus (BLV) infection is a bovine chronic infection caused by BLV, a member of the genus Deltaretrovirus. In this study, we examined the immunomodulatory effects of GS-9620, a toll-like receptor (TLR) 7 agonist, in cattle (Bos taurus) and its therapeutic potential for treating BLV infection. GS-9620 induced cytokine production in peripheral blood mononuclear cells (PBMCs) as well as CD80 expression in CD11c+ cells and increased CD69 and interferon (IFN)-γ expressions in T cells. Removing CD11c+ cells from PBMCs decreased CD69 expression in T cells in the presence of GS-9620. These results suggest that TLR7 agonism promotes T-cell activation via CD11c+ cells. Analyses using PBMCs from BLV-infected cattle revealed that TLR7 expression in CD11c+ cells was upregulated during late-stage BLV infection. Furthermore, GS-9620 increased IFN-γ and TNF-α production and inhibited syncytium formation in vitro, suggesting that GS-9620 may be used to treat BLV infection.

Original languageEnglish
Article number103847
JournalDevelopmental and Comparative Immunology
Volume114
DOIs
Publication statusPublished - 2021 Jan

Keywords

  • Anti-PD-L1 antibody
  • Bovine leukemia virus
  • CD11c cell
  • Cattle
  • T cell
  • TLR7 agonist

ASJC Scopus subject areas

  • Immunology
  • Developmental Biology

Fingerprint Dive into the research topics of 'A TLR7 agonist activates bovine Th1 response and exerts antiviral activity against bovine leukemia virus'. Together they form a unique fingerprint.

Cite this