A three-dimensionalmovie of structural changes in bacteriorhodopsin

Eriko Nango, Antoine Royant, Minoru Kubo, Takanori Nakane, Cecilia Wickstrand, Tetsunari Kimura, Tomoyuki Tanaka, Kensuke Tono, Changyong Song, Rie Tanaka, Toshi Arima, Ayumi Yamashita, Jun Kobayashi, Toshiaki Hosaka, Eiichi Mizohata, Przemyslaw Nogly, Michihiro Sugahara, Daewoong Nam, Takashi Nomura, Tatsuro ShimamuraDohyun Im, Takaaki Fujiwara, Yasuaki Yamanaka, Byeonghyun Jeon, Tomohiro Nishizawa, Kazumasa Oda, Masahiro Fukuda, Rebecka Andersson, Petra Båth, Robert Dods, Jan Davidsson, Shigeru Matsuoka, Satoshi Kawatake, Michio Murata, Osamu Nureki, Shigeki Owada, Takashi Kameshima, Takaki Hatsui, Yasumasa Joti, Gebhard Schertler, Makina Yabashi, Ana Nicoleta Bondar, Jörg Standfuss, Richard Neutze, So Iwata

Research output: Contribution to journalArticlepeer-review

192 Citations (Scopus)

Abstract

Bacteriorhodopsin (bR) is a light-driven proton pump and a model membrane transport protein.We used time-resolved serial femtosecond crystallography at an x-ray free electron laser to visualize conformational changes in bR from nanoseconds to milliseconds following photoactivation. An initially twisted retinal chromophore displaces a conserved tryptophan residue of transmembrane helix F on the cytoplasmic side of the protein while dislodging a key watermolecule on the extracellular side.The resulting cascade of structural changes throughout the protein shows how motions are choreographed as bR transports protons uphill against a transmembrane concentration gradient.

Original languageEnglish
Pages (from-to)1552-1557
Number of pages6
JournalScience
Volume354
Issue number6319
DOIs
Publication statusPublished - 2016 Dec 23
Externally publishedYes

ASJC Scopus subject areas

  • General

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