A technique to enhance electrochemical signals and shorten response time by manipulating yeast near the sensing electrode

Tsung Che Chou, Ching Yu Chang, Kuei Yui Lai, Chi Han Chiou, Chun Hsun Chu, Tomokazu Matsue, Hsien Chang Chang

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

In this work, we manipulated recombinant yeast cells onto a sensing electrode by using hydraulic and electrophoretic force; and thus the current response of trace enzyme activity was increased in p-aminophenyl- β -D - galactopyranoside (PAPG) solution. The recombinant yeast which can generate β-D -galactosidase (β- Gal) in response to steroidal hormone 17 -estradiol were employed as the sensing element of the developed cell-based biosensor. Cells was trapped and stabilized within a microwell structure when they flowed through the sensing electrode. A vertical trapping force was generated by applying +2.0 V across the sensing electrode and an Indium Tin Oxide (ITO). The geometrical electrode area ( 100 × 30 μm2) was defined by a photoresister layer. Since the treated cells were collected on the electrode surface, PAP concentration can be significantly changed around the sensing electrode. Consequently, the detection limit was extend to 0.5 ppb, and the response time was shorten as fast as 15 min by using this device.

Original languageEnglish
Title of host publication2009 IEEE 3rd International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2009
Pages142-145
Number of pages4
DOIs
Publication statusPublished - 2009 Dec 1
Event2009 IEEE 3rd International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2009 - Tainan, Taiwan, Province of China
Duration: 2009 Oct 182009 Oct 21

Other

Other2009 IEEE 3rd International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2009
CountryTaiwan, Province of China
CityTainan
Period09/10/1809/10/21

Keywords

  • Biochip
  • Cell manipulation
  • Cell-based biosensor

ASJC Scopus subject areas

  • Molecular Medicine
  • Pharmacology

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    Chou, T. C., Chang, C. Y., Lai, K. Y., Chiou, C. H., Chu, C. H., Matsue, T., & Chang, H. C. (2009). A technique to enhance electrochemical signals and shorten response time by manipulating yeast near the sensing electrode. In 2009 IEEE 3rd International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2009 (pp. 142-145). [5559100] https://doi.org/10.1109/NANOMED.2009.5559100