A system to induce the deletion of gemomic sequences using R/RS site-specific recombination and the Ac transposon in transgenic rice plants

Y. Nakagawa, C. Machida, Y. Machida, K. Toriyama

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

Recombinase encoded by the R gene of Zygosaccharomyces rouxii mediates reciprocal recombination between two specific recombination sites (RSs) to induce deletion or inversion of the DNA segment that is flanked by the RSs. The R gene under the control of the CaMV 35 S promoter was introduced into rice (Oryza sativa L.). R/RS-specific deletion was first demonstrated in transgenic rice callus carrying the R gene by transient introduction of a cryptic reporter gene that was designed to confer β-glucuronidase (GUS) expression once deletion between two RSs took place. The rice containing the R gene was subsequently crossed with transgenic rice carrying (I-RS/dAc-I-RS) T-DNA that contained RS sequences within the T-DNA and another RS in a modified Ac element that had been transposed to a new locus by Ac transposase. Deletion of the gemomic sequences flanked by the two RSs was detected by PCR analysis in somatic cells of F2 plants. These results demonstrate a technical advance in that the R/RS recombination system, in combination with the Ac transposable element, can be used to generate deletion in rice chromosomes.

Original languageEnglish
Pages (from-to)1136-1141
Number of pages6
JournalTheoretical and Applied Genetics
Volume102
Issue number8
DOIs
Publication statusPublished - 2001 Jul 14

Keywords

  • Deletion
  • Oryza sativa L.
  • R/RS recombination system
  • Site-specific recombination
  • Transposon Ac

ASJC Scopus subject areas

  • Biotechnology
  • Agronomy and Crop Science
  • Genetics

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