A swine toll-like receptor 2-expressing transfectant as a potential primary screening system for immunobiotic microorganisms

Masanori Tohno, Haruki Kitazawa, Takeshi Shimosato, Mayumi Matsumoto, Shinichiro Katoh, Yasushi Kawai, Tadao Saito

    Research output: Contribution to journalArticlepeer-review

    25 Citations (Scopus)

    Abstract

    Toll-like receptor 2 (TLR2) has been shown to mediate cell signaling in response to microbial cell wall components, such as peptidoglycan, lipoteichoic acid, microbial lipoprotein, and zymosan. In this study, we cloned the swine TLR2 and used it to transfect Chinese hamster ovary K-1 cells. We demonstrated that the swine TLR2-expressing transfectant can bind not only zymosan from yeast cell wall components but also intact lactic acid bacteria, resulting in the activation of nuclear factor-κB. These findings suggest that the swine TLR2-expressing transfectant can be very useful for the primary screening of immunobiotic microorganisms.

    Original languageEnglish
    Pages (from-to)283-288
    Number of pages6
    JournalFEMS Immunology and Medical Microbiology
    Volume44
    Issue number3
    DOIs
    Publication statusPublished - 2005 Jun 1

    Keywords

    • Immunobiotic microorganism
    • Immunobiotics
    • Lactic acid bacteria
    • Swine
    • Toll-like receptor 2
    • Transfectant

    ASJC Scopus subject areas

    • Immunology and Allergy
    • Microbiology
    • Immunology
    • Microbiology (medical)
    • Infectious Diseases

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