A swine toll-like receptor 2-expressing transfectant as a potential primary screening system for immunobiotic microorganisms

Masanori Tohno, Haruki Kitazawa, Takeshi Shimosato, Mayumi Matsumoto, Shinichiro Katoh, Yasushi Kawai, Tadao Saito

Research output: Contribution to journalArticlepeer-review

25 Citations (Scopus)

Abstract

Toll-like receptor 2 (TLR2) has been shown to mediate cell signaling in response to microbial cell wall components, such as peptidoglycan, lipoteichoic acid, microbial lipoprotein, and zymosan. In this study, we cloned the swine TLR2 and used it to transfect Chinese hamster ovary K-1 cells. We demonstrated that the swine TLR2-expressing transfectant can bind not only zymosan from yeast cell wall components but also intact lactic acid bacteria, resulting in the activation of nuclear factor-κB. These findings suggest that the swine TLR2-expressing transfectant can be very useful for the primary screening of immunobiotic microorganisms.

Original languageEnglish
Pages (from-to)283-288
Number of pages6
JournalFEMS Immunology and Medical Microbiology
Volume44
Issue number3
DOIs
Publication statusPublished - 2005 Jun 1

Keywords

  • Immunobiotic microorganism
  • Immunobiotics
  • Lactic acid bacteria
  • Swine
  • Toll-like receptor 2
  • Transfectant

ASJC Scopus subject areas

  • Immunology and Allergy
  • Microbiology
  • Immunology
  • Microbiology (medical)
  • Infectious Diseases

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